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Proceedings Paper

Optical mechanisms for detection of lipid-rich atherosclerotic plaques by near-infrared spectroscopy
Author(s): Edward L. Hull; Craig M. Gardner; James E. Muller M.D.; Vianna J. Muller; Christopher V. Salvato; Jennifer B. Lisauskas; Jay D. Caplan
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Paper Abstract

InfraReDx has developed a spectroscopic cardiac catheter system capable of acquiring near-infrared (NIR) reflectance spectra from coronary arteries in vivo for identification of lipid-rich plaques of interest (LRP). The spectral data are analyzed with a chemometric model, producing a hyperspectral image (a chemogram) used to identify LRP in the interrogated region. In this paper, we describe a FT-IR microscopy system for measurement of the NIR scattering and absorption properties of healthy and diseased regions of human coronary arteries in small volumes (~10 μl). Scattering and absorption coefficients are obtained from sequential 140 um x 140 um regions of interest across the face of 500-micron thick, saline-irrigated fresh coronary artery sections. A customized FTIR microscope, measurement protocol, and inversion algorithm are used for optical property determination, and the system is calibrated using measurements of tissue-simulating phantoms having well-characterized optical properties. Tissue optical properties are co-registered with brightfield transmission images as well as with stained histologic thin sections (H&E, Movat Pentachrome, and Oil Red O) acquired from an immediately-adjacent section. The ultimate goal of these experiments is to establish a mechanistic link between the multivariate model predictions displayed on the InfraReDx chemogram and the light-tissue interactions that govern the measured NIR reflectance spectra.

Paper Details

Date Published: 8 February 2008
PDF: 11 pages
Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 68422A (8 February 2008); doi: 10.1117/12.761723
Show Author Affiliations
Edward L. Hull, InfraReDx, Inc. (United States)
Craig M. Gardner, InfraReDx, Inc. (United States)
James E. Muller M.D., InfraReDx, Inc. (United States)
Vianna J. Muller, InfraReDx, Inc. (United States)
Christopher V. Salvato, InfraReDx, Inc. (United States)
Jennifer B. Lisauskas, InfraReDx, Inc. (United States)
Jay D. Caplan, InfraReDx, Inc. (United States)

Published in SPIE Proceedings Vol. 6842:
Photonic Therapeutics and Diagnostics IV
Kenton W. Gregory M.D.; Reza S. Malek M.D.; Nikiforos Kollias; Brian Jet-Fei Wong M.D.; Henry Hirschberg M.D.; Justus F. R. Ilgner M.D.; Steen J. Madsen; Guillermo J. Tearney M.D.; Bernard Choi; Haishan Zeng, Editor(s)

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