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Proceedings Paper

Analysis of caspase3 activation in ChanSu-induced apoptosis of ASTC-a-1 cells by fluorescence techniques
Author(s): Lei Sun; Tongsheng Chen; Longxiang Wang; Huiying Wang
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Paper Abstract

ChanSu(CS), a traditional Chinese medicine, is composed of many chemical compoments. It is isolated from the dried white secretion of the auricular and skin glands of toads, and it has been widely used for treating the heart diseases and other systemic illnesses. However, it is difficult to judge antitumor effect of agents derived from ChanSu and the underlying mechanism of ChanSu inducing cell apoptosis is still unclear. This report was performed to explore the inhibitory effect and mechanism of ChanSu on human lung adenocarcinoma cells (ASTC-a-1). Fluorescence emission spectra and fluorescence resonance energy transfer (FRET) were used to study the caspase-3 activation during the ChanSu-induced human lung adenocarcinoma (ASTC-a-1) cell apoptosis. CCK-8 was used to assay the inhibition of ChanSu on the cell viability. The cells expressing stably with SCAT3 was used to examine if caspase-3 was activated by ChanSu using acceptor photobleaching technique. Our data showed that treatment of ASTC-a-1 cell with ChanSu resulted in the inhibition of viability and induction of apoptosis in a dose-dependent manner and the SCAT3 was almost cleaved 24 h after ChanSu treatment, implying that ChanSu induced cell apoptosis via a caspase-3-dependent death pathway. Our findings extend the knowledge about the cellular signaling mechanisms mediating ChanSu-induced apoptosis.

Paper Details

Date Published: 18 February 2008
PDF: 8 pages
Proc. SPIE 6857, Biophotonics and Immune Responses III, 68570I (18 February 2008); doi: 10.1117/12.761422
Show Author Affiliations
Lei Sun, South China Normal Univ. (China)
Tongsheng Chen, South China Normal Univ. (China)
Longxiang Wang, South China Normal Univ. (China)
Huiying Wang, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 6857:
Biophotonics and Immune Responses III
Wei R. Chen, Editor(s)

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