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Proceedings Paper

Monitoring laser-tissue interaction by non-linear optics
Author(s): Tsung-Hua Tsai; Jin-Ning Lee; Jung-Yi Chan; Jr-Ting Hsu; Hsin-Yuan Tan; Chen-Yuan Dong; Woan-Ruoh Lee; Sung-Jan Lin
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Paper Abstract

To assess laser therapies in clinical practice, histologic examinations were commonly used. But histologic examinations were invasive and not real-time in nature. In this work, we validate multiphoton microscopy as a useful modality in evaluating laser-tissue reaction in vivo. Multiphoton microscopy based on femtosecond titanium-sapphire laser system were used to evaluate autoflurescence (AF) and second harmonic generation (SHG). Nude mouse skin was irradiated with Erbium:YAG laser at low to high fluence. High resolutional images can be obtained by multiphoton microscopy. At low fluence, Erbium:YAG laser can selectively loosen compact stratum corneum with minimal injury to basal layer. At high fluence, ablated keratinocytes and residual debris can be imaged. The laser thermal effect on dermis could be measured by SHG signals of collagen fibers. SHG decreased as laser fluence increased. Multiphoton microscopy is a useful in-vivo technique in evaluating ablative and thermal effects of Erbium:YAG laser on nude mouse skin.

Paper Details

Date Published: 25 February 2008
PDF: 9 pages
Proc. SPIE 6842, Photonic Therapeutics and Diagnostics IV, 684202 (25 February 2008); doi: 10.1117/12.761407
Show Author Affiliations
Tsung-Hua Tsai, Far Eastern Memorial Hospital (Taiwan)
Taipei Medical Univ. (Taiwan)
Jin-Ning Lee, National Taiwan Univ. (Taiwan)
Jung-Yi Chan, Cathay General Hospital (Taiwan)
National Taiwan Univ. Hospital and College of Medicine (Taiwan)
Jr-Ting Hsu, National Taiwan Univ. (Taiwan)
Hsin-Yuan Tan, Chang Gung Memorial Hospital (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)
Woan-Ruoh Lee, Taipei Medical Univ. (Taiwan)
Taipei Medical Univ. Hospital (Taiwan)
Sung-Jan Lin, National Taiwan Univ. Hospital and College of Medicine (Taiwan)
National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6842:
Photonic Therapeutics and Diagnostics IV
Kenton W. Gregory M.D.; Reza S. Malek M.D.; Nikiforos Kollias; Brian Jet-Fei Wong M.D.; Henry Hirschberg M.D.; Justus F. R. Ilgner M.D.; Steen J. Madsen; Guillermo J. Tearney M.D.; Bernard Choi; Haishan Zeng, Editor(s)

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