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Proceedings Paper

Raman spectroscopic study of acute oxidative stress induced changes in mice skeletal muscles
Author(s): Vidyasagar Sriramoju; Alexandra Alimova; Rahul Chakraverty; A. Katz; S. K. Gayen; L. Larsson; H. E. Savage; R. R. Alfano
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Paper Abstract

The oxidative stress due to free radicals is implicated in the pathogenesis of tissue damage in diseases such as muscular dystrophy, Alzheimer dementia, diabetes mellitus, and mitochrondrial myopathies. In this study, the acute oxidative stress induced changes in nicotinamide adenine dinucleotides in mouse skeletal muscles are studied in vitro using Raman spectroscopy. Mammalian skeletal muscles are rich in nicotinamide adenine dinucleotides in both reduced (NADH) and oxidized (NAD) states, as they are sites of aerobic and anaerobic respiration. The relative levels of NAD and NADH are altered in certain physiological and pathological conditions of skeletal muscles. In this study, near infrared Raman spectroscopy is used to identify the molecular fingerprints of NAD and NADH in five-week-old mice biceps femoris muscles. A Raman vibrational mode of NADH is identified in fresh skeletal muscle samples suspended in buffered normal saline. In the same samples, when treated with 1% H2O2 for 5 minutes and 15 minutes, the Raman spectrum shows molecular fingerprints specific to NAD and the disappearance of NADH vibrational bands. The NAD bands after 15 minutes were more intense than after 5 minutes. Since NADH fluoresces and NAD does not, fluorescence spectroscopy is used to confirm the results of the Raman measurements. Fluorescence spectra exhibit an emission peak at 460 nm, corresponding to NADH emission wavelength in fresh muscle samples; while the H2O2 treated muscle samples do not exhibit NADH fluorescence. Raman spectroscopy may be used to develop a minimally invasive, in vivo optical biopsy method to measure the relative NAD and NADH levels in muscle tissues. This may help to detect diseases of muscle, including mitochondrial myopathies and muscular dystrophies.

Paper Details

Date Published: 3 March 2008
PDF: 4 pages
Proc. SPIE 6853, Biomedical Optical Spectroscopy, 685315 (3 March 2008); doi: 10.1117/12.761277
Show Author Affiliations
Vidyasagar Sriramoju, The City College of New York (United States)
Alexandra Alimova, The City College of New York (United States)
Rahul Chakraverty, The City College of New York (United States)
A. Katz, The City College of New York (United States)
S. K. Gayen, The City College of New York (United States)
L. Larsson, Uppsala Univ. (Sweden)
Penn State Univ. (United States)
H. E. Savage, New York Eye and Ear Infirmary (United States)
R. R. Alfano, The City College of New York (United States)

Published in SPIE Proceedings Vol. 6853:
Biomedical Optical Spectroscopy
Anita Mahadevan-Jansen; Robert R. Alfano; Alvin Katz; Wolfgang Petrich, Editor(s)

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