
Proceedings Paper
Recent advances in time-correlated single-photon countingFormat | Member Price | Non-Member Price |
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Paper Abstract
We report about the time-resolved confocal fluorescence microscope MicroTime 200, which is completely based on
TTTR format data acquisition and enables to perform very advanced FCS, FRET and FLIM analysis such as
Fluorescence Lifetime Correlation Spectroscopy (FLCS) or Two Focus FCS (2fFCS).
FLCS is a fundamental improvement of standard FCS overcoming many of its inherent limitations. The basic idea of
FLCS is a weighting of the detected photons based on the additional picosecond timing information (TCSPC start-stop
time) when using pulsed laser excitation. 2fFCS goes even further, combining Pulsed Interleaved Excitation
(PIE) with a time-gated FCS analysis. The basic implementation of 2fFCS uses two synchronized but interleaved
pulsed lasers of the same wavelength but of different polarisation to generate two close by excitation foci in a pre-determined
distance acting as a submicron ruler. In this case it it no longer necessary to have prior knowledge about
the size and shape of the confocal volume. Maintaining the information about the photon´s origin, the dual focus
data allows a precise calculation of absolute diffusion coefficients.
Paper Details
Date Published: 21 February 2008
PDF: 9 pages
Proc. SPIE 6862, Single Molecule Spectroscopy and Imaging, 686209 (21 February 2008); doi: 10.1117/12.761161
Published in SPIE Proceedings Vol. 6862:
Single Molecule Spectroscopy and Imaging
Jörg Enderlein; Zygmunt K. Gryczynski; Rainer Erdmann, Editor(s)
PDF: 9 pages
Proc. SPIE 6862, Single Molecule Spectroscopy and Imaging, 686209 (21 February 2008); doi: 10.1117/12.761161
Show Author Affiliations
Felix Koberling, PicoQuant GmbH (Germany)
Benedikt Krämer, PicoQuant GmbH (Germany)
Sebastian Tannert, PicoQuant GmbH (Germany)
Steffen Rüttinger, PicoQuant GmbH (Germany)
Uwe Ortmann, PicoQuant GmbH (Germany)
Benedikt Krämer, PicoQuant GmbH (Germany)
Sebastian Tannert, PicoQuant GmbH (Germany)
Steffen Rüttinger, PicoQuant GmbH (Germany)
Uwe Ortmann, PicoQuant GmbH (Germany)
Matthias Patting, PicoQuant GmbH (Germany)
Michael Wahl, PicoQuant GmbH (Germany)
Benjamin Ewers, PicoQuant GmbH (Germany)
Peter Kapusta, PicoQuant GmbH (Germany)
Rainer Erdmann, PicoQuant GmbH (Germany)
Michael Wahl, PicoQuant GmbH (Germany)
Benjamin Ewers, PicoQuant GmbH (Germany)
Peter Kapusta, PicoQuant GmbH (Germany)
Rainer Erdmann, PicoQuant GmbH (Germany)
Published in SPIE Proceedings Vol. 6862:
Single Molecule Spectroscopy and Imaging
Jörg Enderlein; Zygmunt K. Gryczynski; Rainer Erdmann, Editor(s)
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