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Proceedings Paper

Determination of glucose in interstitial fluid by surface plasmon resonance biosensor
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Paper Abstract

The concentration of glucose in interstitial fluid determined by using the surface plasmon resonance (SPR) biosensor with chemical bonding D-Galactose/D-Glucose Binding Protein (GGBP) is proposed in this paper. D-Galactose/D-Glucose Binding Protein (GGBP), a kind of protein which has the ability to absorb the glucose specifically, is immobilized on the gold film of the SPR sensor to improve the sensitivity of glucose detecting. The GGBPs mutated at different points have different association abilities with glucose, which bring different measurement range and precision. So the selection of proteins is a critical problem of the determination of glucose by using SPR biosensor. Using different mutated GGBPs, the samples with different concentrations of glucose are measured in the experiment, and the prediction error and precision are discussed. Furthermore, the light intensity of sensor is instable, so the baseline of SPR responses is tracked and adjusted accordingly using the methods - fixing points and fixing areas' ratio. The experiment results show that GGBPs mutated at different points have its corresponding working curves and different measurement precision. In conclusion, the study is significant for the application of SPR biosensor to the minimally invasive diabetes testing and other detection of human body components.

Paper Details

Date Published: 22 February 2008
PDF: 8 pages
Proc. SPIE 6863, Optical Diagnostics and Sensing VIII, 68630M (22 February 2008); doi: 10.1117/12.760894
Show Author Affiliations
Fuxiang Huang, Tianjin Univ. (China)
Jin Liu, Tianjin Univ. (China)
Haixia Yu, Tianjin Univ. (China)
Zengfu Zhang, Tianjin Univ. (China)
Dachao Li, Tianjin Univ. (China)
Kexin Xu, Tianjin Univ. (China)

Published in SPIE Proceedings Vol. 6863:
Optical Diagnostics and Sensing VIII
Gerard L. Coté; Alexander V. Priezzhev, Editor(s)

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