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Proceedings Paper

The NANIVID: a new device for cancer cell migration studies
Author(s): Waseem K. Raja; Nathaniel C. Cady; James Castracane; Bojana Gligorijevic; Jacco van Rheenen; John S. Condeelis
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Paper Abstract

Cancerous tumors are dynamic microenvironments that require unique analytical tools for their study. Better understanding of tumor microenvironments may reveal mechanisms behind tumor progression and generate new strategies for diagnostic marker development, which can be used routinely in histopathological analysis. Previous studies have shown that cell invasion and intravasation are related to metastatic potential and have linked these activities to gene expression patterns seen in migratory and invasive tumor cells in vivo. Existing analytical methods for tumor microenvironments include collection of tumor cells through a catheter needle loaded with a chemical or protein attractant (chemoattractant). This method has some limitations and restrictions, including time constraints of cell collection, long term anesthetization, and in vivo imaging inside the catheter. In this study, a novel implantable device was designed to replace the catheter-based method. The 1.5mm x 0.5mm x 0.24mm device is designed to controllably release chemoattractants for stimulation of tumor cell migration and subsequent cell capture. Devices were fabricated using standard microfabrication techniques and have been shown to mediate controlled release of bovine serum albumin (BSA) and epidermal growth factor (EGF). Optically transparent indium tin oxide (ITO) electrodes have been incorporated into the device for impedance-based measurement of cell density and have been shown to be compatible with in vivo multi-photon imaging of cell migration.

Paper Details

Date Published: 29 February 2008
PDF: 8 pages
Proc. SPIE 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI, 68591M (29 February 2008); doi: 10.1117/12.760730
Show Author Affiliations
Waseem K. Raja, Univ. at Albany (United States)
Nathaniel C. Cady, Univ. at Albany (United States)
James Castracane, Univ. at Albany (United States)
Bojana Gligorijevic, Albert Einstein College of Medicine (United States)
Jacco van Rheenen, Albert Einstein College of Medicine (United States)
John S. Condeelis, Albert Einstein College of Medicine (United States)

Published in SPIE Proceedings Vol. 6859:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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