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Proceedings Paper

Dynamic activation of Src induced by low-power laser irradiation in living cells mediated by reactive oxygen species
Author(s): Juntao Zhang; Xuejuan Gao; Da Xing; Lei Liu
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Paper Abstract

Low-power laser irradiation (LPLI) leads to photochemical reaction and then activates intracellular several signaling pathway. Reactive oxygen species (ROS) are considered to be the primary messengers produced by LPLI. Here, we studied the signaling pathway mediated by ROS upon the stimulation of LPLI. Src tyrosine kinases are well-known targets of ROS and can be activated by oxidative events. Using a Src reporter based on fluorescence resonance energy transfer (FRET) technique, we visualized the dynamic Src activation in Hela cells immediately after LPLI. Moreover, Src activity was enhanced by increasing the duration of LPLI. In addition, our results suggested that ROS were key mediators of Src activation, as ROS scavenger, vitamin C decreased and exogenous H2O2 increased the activity of Src. Meanwhile, Gö6983 loading did not block the effect of LPLI. CCK-8 experiments proved that cell vitality was prominently improved by LPLI with all the doses we applied in our experiments ranging from 3 to 25J/cm2. The results indicated that LPLI/ROS/Src pathway may be involved in the LPLI biostimulation effects.

Paper Details

Date Published: 11 January 2008
PDF: 9 pages
Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 68260K (11 January 2008); doi: 10.1117/12.760229
Show Author Affiliations
Juntao Zhang, South China Normal Univ. (China)
Xuejuan Gao, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Lei Liu, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 6826:
Optics in Health Care and Biomedical Optics III
Xingde Li; Qingming Luo; Ying Gu M.D., Editor(s)

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