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Proceedings Paper

Multiplexed BioCD for prostate specific antigen detection
Author(s): Xuefeng Wang; Ming Zhao; David D. Nolte
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Paper Abstract

Specific protein concentrations in human body fluid can serve as diagnostic markers for some diseases, and a quantitative and high-throughput technique for multiplexed protein detection would speed up diagnosis and facilitate medical research. For this purpose, our group developed the BioCD, a spinning-disc interferometric biosensor on which antibody is immobilized. The detection system adopts a common-path scheme making it ultra stable. The scaling mass sensitivity is below 10 pg/mm for protein surface density. A 25000-spot antibody BioCD was fabricated to measure the concentration of prostate specific antigen (PSA), a protein indicating prostate cancer if its level is high. Statistical analysis of our immunoassay results projects that the detection limit of PSA would reach 20 pg/ml in a 2 mg/ml background solution. For future prospects, a multiplexed BioCD can be produced for simultaneous diagnosis of diverse diseases. For instance, 100 markers above 200 pg/ml could be measured on a single disc given that the detection limit is inversely proportional to square root of the number of spots.

Paper Details

Date Published: 12 February 2008
PDF: 8 pages
Proc. SPIE 6848, Advanced Biomedical and Clinical Diagnostic Systems VI, 68480S (12 February 2008); doi: 10.1117/12.759173
Show Author Affiliations
Xuefeng Wang, Purdue Univ. (United States)
Ming Zhao, Purdue Univ. (United States)
David D. Nolte, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 6848:
Advanced Biomedical and Clinical Diagnostic Systems VI
Tuan Vo-Dinh; Warren S. Grundfest; David A. Benaron; Gerald E. Cohn, Editor(s)

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