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Proceedings Paper

Measurement of intracellular pH (pHi) in a single cell using fluorescent probe and fiber optic nanoprobe
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Paper Abstract

An optical system for measurement of the intracellular pH (pHi) in a single living cell by using the fluorescent probe, 5(6)-carboxyfluorescein diacetate (CFDA) and fiber optic nanoprobe was demonstrated in this work. The CFDA probe is used to determine pHi in the yeast, Saccharomyces cerevisiae 97 while fiber optic nanoprobe is used to guide excitation light and receive emission light within a single cell. Experimental results showed that our system had higher detection sensitivity than other standard spectrometer, which is important to single-cell analysis, especially for the microanalysis in a single-cell.

Paper Details

Date Published: 8 January 2008
PDF: 6 pages
Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 68262N (8 January 2008); doi: 10.1117/12.755230
Show Author Affiliations
Qinmiao Chen, Fujian Normal Univ. (China)
Yishen Qiu, Fujian Normal Univ. (China)
Zhihao Chen, Institute for Infocomm Research (Singapore)
Na Fang, Fujian Normal Univ. (China)
Gaoming Li, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 6826:
Optics in Health Care and Biomedical Optics III
Xingde Li; Qingming Luo; Ying Gu M.D., Editor(s)

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