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Proceedings Paper

Diffuse fluorescence tomography of exo- and endogenously labeled tumors
Author(s): Irina V. Balalaeva; Ilya V. Turchin; Anna G. Orlova; Vladimir I. Plekhanov; Marina V. Shirmanova; Michail S. Kleshnin; Ilya I. Fiks; Elena V. Zagainova; Vladislav A. Kamensky
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Paper Abstract

Strong light scattering and absorption limit observation of the internal structure of biological tissue. Only special tools for turbid media imaging, such as optical diffuse tomography, enable noninvasive investigation of the internal biological tissues, including visualization and intravital monitoring of deep tumors. In this work the preliminary results of diffuse fluorescence tomography (DFT) of small animals are presented. Usage of exogenous fluorophores, targeted specifically at tumor cells, and fluorescent proteins expressed endogenously can significantly increase the contrast of obtained images. Fluorescent compounds of different nature, such as sulphonated aluminium phthalocyanine (Photosens), red fluorescing proteins and CdTe/CdSe-core/shell nanocrystals (quantum dots) were applied. We tested diffuse fluorescence tomography method at model media, in post mortem and in vivo experiments. The animal was scanned in transilluminative configuration by low-frequency modulated light (1 kHz) from Nd:YAG laser with second harmonic generation at wavelength of 532 nm or semiconductor laser at wavelength of 655 nm. Quantum dots or protein DsRed2 in glass capsules (inner diameter 2-3 mm) were placed post mortem inside the esophagus of 7-day-old hairless rats to simulate marked tumors. Photosens was injected intravenously to linear mice with metastazing Lewis lung carcinoma. The reconstruction algorithm, based on Algebraic Reconstruction Technique, was created and tested numerically in model experiments. High contrast images of tumor simulating capsules with DsRed2 concentrations about 10-6 M and quantum dots about 5x10-11 M have been obtained. Organ distribution of Photosens and its accumulation in tumors and surrounding tissues of animals has been examined. We have conducted the monitoring of tumors, exogenously labeled by photosensitizer. This work demonstrates potential capabilities of DFT method for intravital detection and monitoring of deep fluorescent-labeled tumors in animal models. The comparative analysis of conventional photosensitizer, fluorescent proteins and quantum dots has been carried out.

Paper Details

Date Published: 1 August 2007
PDF: 8 pages
Proc. SPIE 6734, International Conference on Lasers, Applications, and Technologies 2007: Laser Technologies for Medicine, 67340K (1 August 2007); doi: 10.1117/12.753220
Show Author Affiliations
Irina V. Balalaeva, Institute of Applied Physics (Russia)
Nizhny Novgorod State Univ. (Russia)
Ilya V. Turchin, Institute of Applied Physics (Russia)
Anna G. Orlova, Institute of Applied Physics (Russia)
Vladimir I. Plekhanov, Institute of Applied Physics (Russia)
Marina V. Shirmanova, Nizhny Novgorod State Univ. (Russia)
Institute of Fundamental and Applied Medicine (Russia)
Michail S. Kleshnin, Institute of Applied Physics (Russia)
Ilya I. Fiks, Institute of Applied Physics (Russia)
Elena V. Zagainova, Institute of Fundamental and Applied Medicine (Russia)
Vladislav A. Kamensky, Institute of Applied Physics (Russia)

Published in SPIE Proceedings Vol. 6734:
International Conference on Lasers, Applications, and Technologies 2007: Laser Technologies for Medicine
Vladislav Panchenko; Andrey Larichev; George Zheltov, Editor(s)

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