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Proceedings Paper

A novel method for rapid and non-invasive detection of plants senescence using delayed fluorescence technique
Author(s): Lingrui Zhang; Da Xing; Junsheng Wang; Lizhang Zeng; Qiang Li
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Paper Abstract

Plants senescence is a phase of plants ontogeny marked by declining photosynthetic activity that is paralleled by a decline in chloroplast function. The photosystem II ( PSII ) in a plant is considered the primary site where light-induced delayed fluorescence (DF) is produced. With the leaves of Catharanthus roseus (Catharanthus roseus (L.) G.Don) as testing models, we have studied the effects of plants senescence induced by dark and/or exogenous hormones treatments on characteristics of DF by using a home-made portable DF detection system, which can enable various DF parameters, such as DF decay kinetic curve and DF intensity, to be rapidly produced for the plants in a short time. The results show that the changes in DF intensity of green plants can truly reflect the changes in photosynthetic capacity and chlorophyll content. Therefore, DF may be used an important means of evaluating in vivo plants senescence physiology. The changes in DF intensity may provide a new approach for the rapid and early detection of plants senescence caused by age or other senescence-related factors. DF technique could be potential useful for high throughput screening and less time-consuming and automated identifying the interesting mutants with genetic modifications that change plants senescence progress.

Paper Details

Date Published: 1 May 2007
PDF: 8 pages
Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 653427 (1 May 2007); doi: 10.1117/12.741637
Show Author Affiliations
Lingrui Zhang, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Junsheng Wang, South China Normal Univ. (China)
Lizhang Zeng, South China Normal Univ. (China)
Qiang Li, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 6534:
Fifth International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin; Min Gu, Editor(s)

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