Share Email Print

Proceedings Paper

Membranotropic photobiomodulation on red blood cell deformability
Author(s): Gang-Yue Luo; Yan-Ping Zhao; Timon Cheng-Yi Liu; Song-Hao Liu
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

To assess modulation of laser on erythrocyte permeability and deformability via cell morphology changes, healthy human echinocytes with shrinking size and high plasmic viscosity due to cellular dehydration were treated with 1 mW, 2 mW, 3 mW, and 5 mW laser power exposure respectively. Image analyzing system on single intact erythrocyte was applied for measuring comprehensive cell morphological parameters (surface area, external membrane perimeter, circle index and elongation index) that were determined by the modulation of erythrocyte water permeability and deformability to detect relationship between erythrocyte water permeability alteration and deformability. Our preliminary experiment showed that exposure under light dose of 5 mW for 5 min could induce more active erythrocyte swelling and deformation. water channel aquaporin-1(AQP-1) was inhibited by the incubation of HgCl2 in the presence and absence of 5 mW laser irradiation. The result suggested that osmotic water permeability is a primary factor in the procedure of erythrocyte deformability. In addition, no modulation of laser(5mW) on erythrocyte deformability had been found when the echinocytes were cultured with GDP-β-S (G protein inhibitor).

Paper Details

Date Published: 1 May 2007
PDF: 11 pages
Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 653424 (1 May 2007); doi: 10.1117/12.741631
Show Author Affiliations
Gang-Yue Luo, South China Normal Univ. (China)
Yan-Ping Zhao, South China Normal Univ. (China)
Timon Cheng-Yi Liu, South China Normal Univ. (China)
Song-Hao Liu, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 6534:
Fifth International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin; Min Gu, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?