
Proceedings Paper
High sensitive method detection of plant RNA viruses by electrochemiluminescence reverse transcription PCRFormat | Member Price | Non-Member Price |
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Paper Abstract
It is well known that plant and animal viruses had widely spread the whole of world, and made a big loss in farming and
husbandry. It is necessary that a highly efficient and accurate virus's detection method was developed. This research
combines reverse transcription polymerase chain reaction (RT-PCR) technique with electrochemiluminescence method,
to detect plant RNA viruses for the first time. Biotin-probe hybridizes with PCR product to specific select the target for
detection, thus can avoid pseudo-positive result. TBR-probe hybridizes with PCR product to emit light for ECL detection.
Specific nucleic acid sequences (20bp) were added to 5' terminal all of the primers, which can improve the chance of
hybridization between TBR-probe and PCR product. At the same time, one of the PCR product chain can hybridize two
Ru-probes, the ECL signal is intensified. The method was used to detect Odntoglossum ringspot virus ORSV, Sugarcane
mosaic virus ScMV, Sorghum mosaic virus SrMV, and Maize dwarf mosaic virus MDMV, the experiment results show
that this method could reliably identity virus infected plant samples. In a word, this method has higher sensitivity and
lower cost than others. It can effectively detect the plant viruses with simplicity, stability, and high sensitivity.
Paper Details
Date Published: 1 May 2007
PDF: 8 pages
Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65341E (1 May 2007); doi: 10.1117/12.741573
Published in SPIE Proceedings Vol. 6534:
Fifth International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin; Min Gu, Editor(s)
PDF: 8 pages
Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65341E (1 May 2007); doi: 10.1117/12.741573
Show Author Affiliations
Published in SPIE Proceedings Vol. 6534:
Fifth International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin; Min Gu, Editor(s)
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