Proceedings Paper
Dynamic imaging of cerebral blood flow in rat reperfused mini-stroke model using laser speckle temporal contrast analysis| Format | Member Price | Non-Member Price |
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Paper Abstract
Laser speckle temporal contrast analysis (LSTCA) was used to image the cerebral blood flow (CBF) of ischemic area in
reperfused mini-stroke model in rats. Focal cortical ischemia in male Sprague-Dawley rats (n=20) was induced by
deliberate ligation of multiple branches of the middle cerebral artery (MCA) together with a nylon ring and the dura.
LSTCA was used to monitor the spatio-temporal characteristics of cerebral blood flow dynamics in the rat
somatosensory cortex in the ischemic and reperfused stages. The infarction volume was measured by 2, 3, 5-
triphenyltetrazolium chloride (TTC) staining 24 hours after reperfusion. The distribution of changes in cerebral blood
flow which outlined by the laser speckle imaging represented the relative CBF gradient (21.98±1.96%, 67.2±1.67 %,
107.24±4.71 % of the baseline) from ischemic core, penumbra zone to normal tissue immediately after cortical
ischemia, in which a central ischemic core had little or no perfusion surrounded by a penumbral region with reduced
perfusion, in addition, we had shown the existence of a surrounding region of hyperemic tissue; Thereafter a postrecanalization
hyperperfusion occurred in the same infarct core since 24 hours after reperfusion (242.62±18.52% of the
baseline). Histology of the ischemic regions at 24 hours after reperfusion revealed small focal infarcts that were
typically 3~4 mm in diameter, approximately equal to the nylon ring in size and position and essentially accordant with
the spatial distribution of the ischemic cortex with below 30% residual CBF of the pre-ischemic baseline. It was
demonstrated that this technique of LSTCA was easy to implement and availably used to image the spatial and temporal
evolution of CBF changes with high resolution in rat reperfused mini-stroke model.
Paper Details
Date Published: 1 May 2007
PDF: 8 pages
Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65343N (1 May 2007); doi: 10.1117/12.741504
Published in SPIE Proceedings Vol. 6534:
Fifth International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin; Min Gu, Editor(s)
PDF: 8 pages
Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65343N (1 May 2007); doi: 10.1117/12.741504
Show Author Affiliations
Zhen Wang, The Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Weihua Luo, The Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Pengcheng Li, The Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Weihua Luo, The Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Pengcheng Li, The Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, The Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Qingming Luo, The Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Qingming Luo, The Key Lab. of Biomedical Photonics, Huazhong Univ. of Science and Technology (China)
Published in SPIE Proceedings Vol. 6534:
Fifth International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin; Min Gu, Editor(s)
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