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Proceedings Paper

Frequency domain fluorescence diffuse tomography of small animals
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Paper Abstract

Fluorescent compounds for selective cancer cell marking are used for development of novel medical diagnostic methods, investigation of the influence of external factors on tumor growth, regress and metastasis. Only special tools for turbid media imaging, such as optical diffusion tomography permit noninvasive monitoring of fluorescent-labeled tumor alterations deep in animal tissue. In this work, the results of preliminary experiments utilizing frequency-domain fluorescent diffusion tomography (FD FDT) experimental setup in small animal are presented. Low-frequency modulated light (1 kHz) from Nd:YAG laser with second harmonic generation at the wavelength of 532 nm was used in the setup. The transilluminative planar configuration was used in the setup. A series of model experiments has been conducted and show good agreement between theoretical and experimental fluorescence intensity. Models of deep tumors were created by two methods: (1) glass capsules containing fluorophore solution were inserted into esophagus of small animals to simulate marked tumors; (2) a suspension of transfected HE&Kgr;293-Turbo-RFP cells was subcutaneously injected to small animal. The conducted experiments have shown that FD FDT allows one to detect the presence of labeled tumor cells in small animals, to determine the volume of an experimental tumor, to perform 3D tumor reconstruction, as well as to conduct monitoring investigations. The obtained results demonstrate the potential capability of the FD FDT method for noninvasive whole-body imaging in cancer studies, diagnostics and therapy.

Paper Details

Date Published: 1 May 2007
PDF: 8 pages
Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65343G (1 May 2007); doi: 10.1117/12.741469
Show Author Affiliations
Anna G. Orlova, Institute of Applied Physics (Russia)
Ilya V. Turchin, Institute of Applied Physics (Russia)
Vladislav A. Kamensky, Institute of Applied Physics (Russia)
Vladimir I. Plehanov, Institute of Applied Physics (Russia)
Irina V. Balalaeva, Institute of Applied Physics (Russia)
Ekaterina A. Sergeeva, Institute of Applied Physics (Russia)
Marina V. Shirmanova, Institute of Applied Physics (Russia)
Michail S. Kleshnin, Institute of Applied Physics (Russia)

Published in SPIE Proceedings Vol. 6534:
Fifth International Conference on Photonics and Imaging in Biology and Medicine
Qingming Luo; Lihong V. Wang; Valery V. Tuchin; Min Gu, Editor(s)

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