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Proceedings Paper

Laser microbeams and optical tweezers to study DNA repair and ageing
Author(s): Paulius Grigaravicius; Shamci Monajembashi; Götz Pilarczyk; Alexander Rapp; Karl Otto Greulich
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Paper Abstract

Incorrect DNA repair is probably one cause of healthy ageing. Laser microbeams or optical tweezers are emerging as convenient tools in the study of repair mechanisms. Using such tools, DNA damage can be induced in a preselected volume element of a cell nucleus and at a preselected time point - an effect which is hardly to achieve with any other tool. On the other hand damage induction highly depends on a subtle combination of laser mircobeam parameters such as dose, pulse peak power and wavelength. In consequence DNA repair at the sites of damage may work differently. Furthermore, such sites are occasionally stationary, occasionally they migrate towards each other, indicating a considerable dynamics of DNA repair inside a cell nucleus. As an example for the application of optical tweezers, Erythrocyte Mediated Force Application (EMFA) is used to induce nitric oxide production in cells of the endothelium, i. e. the inner layer of (human) blood vessels. It is shown that upon stimulation by EMFA, endothelial cells initially activate the calcium homeostasis and develop calcium humps, concentration plateaus and oscillations.

Paper Details

Date Published: 14 September 2007
PDF: 12 pages
Proc. SPIE 6644, Optical Trapping and Optical Micromanipulation IV, 66440J (14 September 2007); doi: 10.1117/12.735816
Show Author Affiliations
Paulius Grigaravicius, Fritz Lipmann Institute (Germany)
Shamci Monajembashi, Fritz Lipmann Institute (Germany)
Götz Pilarczyk, Univ. of Giessen (Germany)
Alexander Rapp, Fritz Lipmann Institute (Germany)
Univ. of Oxford (United Kingdom)
Karl Otto Greulich, Fritz Lipmann Institute (Germany)

Published in SPIE Proceedings Vol. 6644:
Optical Trapping and Optical Micromanipulation IV
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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