
Proceedings Paper
Optical tweezers and multiphoton microscopies integrated photonic tool for mechanical and biochemical cell processes studiesFormat | Member Price | Non-Member Price |
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Paper Abstract
The research in biomedical photonics is clearly evolving in the direction of the understanding of biological
processes at the cell level. The spatial resolution to accomplish this task practically requires photonics tools.
However, an integration of different photonic tools and a multimodal and functional approach will be necessary to
access the mechanical and biochemical cell processes. This way we can observe mechanicaly triggered biochemical
events or biochemicaly triggered mechanical events, or even observe simultaneously mechanical and biochemical
events triggered by other means, e.g. electricaly. One great advantage of the photonic tools is its easiness for
integration. Therefore, we developed such integrated tool by incorporating single and double Optical Tweezers with
Confocal Single and Multiphoton Microscopies. This system can perform 2-photon excited fluorescence and Second
Harmonic Generation microscopies together with optical manipulations. It also can acquire Fluorescence and SHG
spectra of specific spots. Force, elasticity and viscosity measurements of stretched membranes can be followed by
real time confocal microscopies. Also opticaly trapped living protozoas, such as leishmania amazonensis.
Integration with CARS microscopy is under way. We will show several examples of the use of such integrated
instrument and its potential to observe mechanical and biochemical processes at cell level.
Paper Details
Date Published: 5 September 2007
PDF: 8 pages
Proc. SPIE 6644, Optical Trapping and Optical Micromanipulation IV, 66440H (5 September 2007); doi: 10.1117/12.734495
Published in SPIE Proceedings Vol. 6644:
Optical Trapping and Optical Micromanipulation IV
Kishan Dholakia; Gabriel C. Spalding, Editor(s)
PDF: 8 pages
Proc. SPIE 6644, Optical Trapping and Optical Micromanipulation IV, 66440H (5 September 2007); doi: 10.1117/12.734495
Show Author Affiliations
A. A. de Thomaz, State Univ. of Campinas (Brazil)
W. M. Faustino, State Univ. of Campinas (Brazil)
A. Fontes, Federal Univ. of Pernambuco (Brazil)
H. P. Fernandes, State Univ. of Campinas (Brazil)
M. d. L. Barjas-Castro, State Univ. of Campinas (Brazil)
W. M. Faustino, State Univ. of Campinas (Brazil)
A. Fontes, Federal Univ. of Pernambuco (Brazil)
H. P. Fernandes, State Univ. of Campinas (Brazil)
M. d. L. Barjas-Castro, State Univ. of Campinas (Brazil)
K. Metze, State Univ. of Campinas (Brazil)
S. Giorgio, State Univ. of Campinas (Brazil)
L. C. Barbosa, State Univ. of Campinas (Brazil)
C. L. Cesar, State Univ. of Campinas (Brazil)
S. Giorgio, State Univ. of Campinas (Brazil)
L. C. Barbosa, State Univ. of Campinas (Brazil)
C. L. Cesar, State Univ. of Campinas (Brazil)
Published in SPIE Proceedings Vol. 6644:
Optical Trapping and Optical Micromanipulation IV
Kishan Dholakia; Gabriel C. Spalding, Editor(s)
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