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Proceedings Paper

Wide-field photon counting imaging for fluorescence microscopy
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Paper Abstract

We present a novel time-resolved photon counting imaging technique and its use in multi-dimensional luminescence spectroscopy. By using an ultrafast camera coupled to an image intensifier on a microscope, we demonstrate the potential of wide-field time-correlated single photon counting, with a count rate of up to 5 Mhz. This system has the advantage of allowing the detection of single photons in parallel in every pixel. We measured the luminescence decay of Europium Polyoxometalate (POM), and observed contrast on lifetime images of Eu-POM on silver nanocrystals.

Paper Details

Date Published: 15 October 2007
PDF: 8 pages
Proc. SPIE 6771, Advanced Photon Counting Techniques II, 67710X (15 October 2007); doi: 10.1117/12.733793
Show Author Affiliations
Nicolas Sergent, King's College London (United Kingdom)
James A. Levitt, King's College London (United Kingdom)
Mark A. Green, King's College London (United Kingdom)
Klaus Suhling, King's College London (United Kingdom)

Published in SPIE Proceedings Vol. 6771:
Advanced Photon Counting Techniques II
Wolfgang Becker, Editor(s)

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