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Proceedings Paper

Supported lipid bilayer membranes on SiO2 and TiO2: substrate effects on membrane formation and shape transformation
Author(s): Ryugo Tero; Toru Ujihara; Tsuneo Urisu
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Paper Abstract

Lipid bilayer membranes deposited on solid surfaces are called "supported planar bilayers" (SPBs), and expected to be an effective cell-membrane-mimicking model system in vitro. We have investigated the influence of the substrate surface properties on the SPB formation process and on the photo-induced shape transformation of bilayers, by means of atomic force microscopy and fluorescence microscopy. The SPB of dipalmitoleoylphosphatidylcholine was formed on SiO2/Si(100) surfaces and rutile-TiO2(100) surface by the vesicle fusion method. On the SiO2 surface, one or a few adsorbed vesicles can transform to a SPB resulting in a small bilayer patches. The SPB formation rate was accelerated on thermally treated SiO2 surfaces, which had less hydrophilicity, but the initial SPB formation process did not change. On the TiO2(100), the surface was completely covered with the adsorbed vesicles prior to the SPB formation, and the planar bilayer was obtained only if the lipid concentration in the suspension was sufficiently high. Photo-induced activation of molecular motion through the fluorescence dye excitation achieved the area-selective SPB formation from the adsorbed vesicular layers with small SPB domains on the TiO2(100). This photo-activation transformed the SPB shapes threedimensionally on the SiO2 and TiO2 surfaces in different way.

Paper Details

Date Published: 25 September 2007
PDF: 12 pages
Proc. SPIE 6769, Nanosensing: Materials, Devices, and Systems III, 67690J (25 September 2007); doi: 10.1117/12.733628
Show Author Affiliations
Ryugo Tero, Institute for Molecular Science (Japan)
Toru Ujihara, Nagoya Univ. (Japan)
Tsuneo Urisu, Institute for Molecular Science (Japan)

Published in SPIE Proceedings Vol. 6769:
Nanosensing: Materials, Devices, and Systems III
M. Saif Islam; Achyut K. Dutta, Editor(s)

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