
Proceedings Paper
SERS beacons for multiplexed oligonucleotide detectionFormat | Member Price | Non-Member Price |
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Paper Abstract
Gold-based surface-enhanced Raman scattering (SERS) beacons have been developed, which represent a
simple, biocompatible and rapid means of performing multiplexed DNA sequence detection in a non-arrayed format.
These SERS beacons consist of a simple stem-loop oligonucleotide probe in its native form with one end attached to
a SERS active dye molecule and the other to a gold nanoparticle, approximately 50 nm in diameter. The probe
sequence is designed to achieve a stem-loop structure, with the loop portion complementary to the target sequence,
similar to fluorescent molecular beacons. In the absence of the target DNA sequence, the SERS signal of the
associated dye molecule is detected, representing the "ON" state of the probe. When the target sequence is
hybridized to the probe, which results in an open conformation, its respective reporter dye is separated from the gold
nanoparticle, producing diminished SERS signal. In this paper, the fabrication and characterization of these SERS
beacons is described. We also demonstrate selective hybridization of a target sequence to one beacon in a mixture,
revealing their potential for use in a multiplexed fashion.
Paper Details
Date Published: 4 October 2007
PDF: 8 pages
Proc. SPIE 6759, Smart Biomedical and Physiological Sensor Technology V, 67590F (4 October 2007); doi: 10.1117/12.730450
Published in SPIE Proceedings Vol. 6759:
Smart Biomedical and Physiological Sensor Technology V
Brian M. Cullum; D. Marshall Porterfield, Editor(s)
PDF: 8 pages
Proc. SPIE 6759, Smart Biomedical and Physiological Sensor Technology V, 67590F (4 October 2007); doi: 10.1117/12.730450
Show Author Affiliations
Jian Sun, Univ. of Maryland/Baltimore County (United States)
Brian M. Cullum, Univ. of Maryland/Baltimore County (United States)
Published in SPIE Proceedings Vol. 6759:
Smart Biomedical and Physiological Sensor Technology V
Brian M. Cullum; D. Marshall Porterfield, Editor(s)
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