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Proceedings Paper

Sensing metabolic activity in tissue engineered constructs
Author(s): Malavika Chandra; Robert H. Wilson; Wen-Liang Lo; Karthik Vishwanath; Kenji Izumi; Stephen E. Feinberg; Mary-Ann Mycek
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Paper Abstract

Tissue engineered constructs can be employed to graft wounds or replace diseased tissue. Non-invasive methods are required to assess cellular viability in these constructs both pre- and post-implantation into patients. In this study, Monte Carlo simulations and fluorescence experiments were executed on ex vivo produced oral mucosa equivalent (EVPOME) constructs to investigate the fluorescence emitted at 355 nm excitation from these constructs. Both simulations and experiments indicated the need to investigate alternative excitation wavelengths in order to increase the cellular fluorescence from these constructs, while decreasing contributions from extra-cellular fluorophores.

Paper Details

Date Published: 11 July 2007
PDF: 7 pages
Proc. SPIE 6628, Diagnostic Optical Spectroscopy in Biomedicine IV, 66280B (11 July 2007); doi: 10.1117/12.729426
Show Author Affiliations
Malavika Chandra, Univ. of Michigan (United States)
Robert H. Wilson, Univ. of Michigan (United States)
Wen-Liang Lo, Univ. of Michigan (United States)
Karthik Vishwanath, Univ. of Michigan (United States)
Kenji Izumi, Univ. of Michigan (United States)
Stephen E. Feinberg, Univ. of Michigan (United States)
Mary-Ann Mycek, Univ. of Michigan (United States)
Univ. of Michigan Medical School (United States)

Published in SPIE Proceedings Vol. 6628:
Diagnostic Optical Spectroscopy in Biomedicine IV
Dietrich Schweitzer; Maryann Fitzmaurice, Editor(s)

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