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Proceedings Paper

Spherical aberration cancellation in a polarized photon-pairs confocal laser scanning microscope
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Paper Abstract

Refractive-index mismatch in conventional confocal microscopy produces severe degradation on axial resolution of sectioning image because the spherical aberration is generated in specimen. In this study, we propose a polarized photon-pairs confocal laser scanning microscope (PCLSM) in which a two-frequency linear polarized photon-pairs (LPPPs) laser beam is produced. The common-path propagation of LPPPs integrated with optical heterodyne technique not only can reduce the spherical aberration but also decreases scattering effect in specimen at same time. Therefore, the better axial and lateral resolutions of the sectioning image are produced simultaneously. In the experiment, a verification and comparison between PCLSM and conventional confocal laser scanning microscope (CLSM) on the ability of cancellation of spherical aberration induced by cover glass are demonstrated experimentally. Finally, the ability of PCLSM which can decrease the spherical aberration based on the common-path propagation of LPPPs associated with polarization gating, spatial coherence gating and spatial filtering gating is discussed.

Paper Details

Date Published: 12 July 2007
PDF: 9 pages
Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 66300N (12 July 2007); doi: 10.1117/12.728593
Show Author Affiliations
Chi-Hui Chang, National Central Univ. (Taiwan)
Chien Chou, National Central Univ. (Taiwan)
National Yang Ming Univ. (Taiwan)
Hsiu-Fong Chang, National Central Univ. (Taiwan)
Science and Technology Institute of Northern Taiwan (Taiwan)
Hon-Fai Yau, National Central Univ. (Taiwan)
Huan-Jang Huang, National Yang Ming Univ. (Taiwan)
Wen-Chuan Kuo, National Taiwan Normal Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6630:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging III
Tony Wilson; Ammasi Periasamy, Editor(s)

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