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Proceedings Paper

Highly sensitive detection of target molecules using a new fluorescence-based bead assay
Author(s): Silvia Scheffler; Denis Strauß; Markus Sauer
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Paper Abstract

Development of immunoassays with improved sensitivity, specificity and reliability are of major interest in modern bioanalytical research. We describe the development of a new immunomagnetic fluorescence detection (IM-FD) assay based on specific antigen/antibody interactions and on accumulation of the fluorescence signal on superparamagnetic PE beads in combination with the use of extrinsic fluorescent labels. IM-FD can be easily modified by varying the order of coatings and assay conditions. Depending on the target molecule, antibodies (ABs), entire proteins, or small protein epitopes can be used as capture molecules. The presence of target molecules is detected by fluorescence microscopy using fluorescently labeled secondary or detection antibodies. Here, we demonstrate the potential of the new assay detecting the two tumor markers IGF-I and p53 antibodies in the clinically relevant concentration range. Our data show that the fluorescence-based bead assay exhibits a large dynamic range and a high sensitivity down to the subpicomolar level.

Paper Details

Date Published: 13 July 2007
PDF: 8 pages
Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66332D (13 July 2007); doi: 10.1117/12.728462
Show Author Affiliations
Silvia Scheffler, Bielefeld Univ. (Germany)
Denis Strauß, Bielefeld Univ. (Germany)
Markus Sauer, Bielefeld Univ. (Germany)

Published in SPIE Proceedings Vol. 6633:
Biophotonics 2007: Optics in Life Science
Jürgen Popp; Gert von Bally, Editor(s)

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