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Proceedings Paper

Protein chip analysis by probing time-resolved UV fluorescence
Author(s): Paulius Grigaravicius; Rüdiger Dietrich; Wolfgang Fritzsche; Karl Otto Greulich; Uwe Horn; Dietmar Knoll; Sven Peters; Hans-Martin Striebel; Peter Schellenberg
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Paper Abstract

We describe a novel label-free method to analyse protein interactions on microarrays as well as in solution. By this technique the time resolved native protein fluorescence in the UV is probed. The method is based on alterations of the protein upon ligand binding, and, as a consequence, of alterations of the environment of the proteins' aromatic amino acids. These amino acids act as internal probes, and as a result, the fluorescence lifetime of the proteins change due to binding to a ligand partner such as another protein. We were able to demonstrate the feasibility of the method with many compounds, including protein-protein, protein-antibody, protein-nucleic acid and protein-small ligand pairs. Unlike to many other label-free techniques, the sensitivity of the method does not depend on the size of the counterbinding ligand and therefore is particularly suitable for drug monitoring, when small molecules are involved.

Paper Details

Date Published: 13 July 2007
PDF: 7 pages
Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66332E (13 July 2007); doi: 10.1117/12.728451
Show Author Affiliations
Paulius Grigaravicius, Leibniz Institute for Age Research, Fritz Lipmann Institute (Germany)
Rüdiger Dietrich, Schott Jenaer Glass GmbH (Germany)
Wolfgang Fritzsche, Institute of Photonic Technology (Germany)
Karl Otto Greulich, Leibniz Institute for Age Research, Fritz Lipmann Institute (Germany)
Uwe Horn, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (Germany)
Dietmar Knoll, Schott Jenaer Glass GmbH (Germany)
Sven Peters, Institute of Photonic Technology (Germany)
Hans-Martin Striebel, Leibniz Institute for Age Research, Fritz Lipmann Institute (Germany)
Peter Schellenberg, Institute of Photonic Technology (Germany)

Published in SPIE Proceedings Vol. 6633:
Biophotonics 2007: Optics in Life Science
Jürgen Popp; Gert von Bally, Editor(s)

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