
Proceedings Paper
Synthesis, functionalization, and characterization of rod-shaped gold nanoparticles as potential optical contrast agentsFormat | Member Price | Non-Member Price |
---|---|---|
$17.00 | $21.00 |
Paper Abstract
Gold nanoparticles exhibit intense and narrow optical extinction bands due to the phenomenon of plasmon resonance
making them useful as contrast agents for light-based imaging techniques. Localized heating results from the absorbed
light energy, which shows potential for these particles in photothermal therapy as well. The bioconjugation of gold
nanoparticles to appropriate antibodies targeted to tumors in vivo, could make highly selective detection and therapy of
tumors possible. We have synthesised gold nanorods based on seed mediated protocols using two methods. The first
method is based on using a mono-surfactant silver assisted method which produces gold nanorods having plasmon peaks
between 670-850 nm within the "optical imaging and therapeutic window". These nanorods have aspect ratios between
2.3 - 3.7. A second method is a silver assisted bi-surfactant method which produce nanorods with peaks in the range of
850-1100 nm having aspect ratios between 5 - 11. Typical concentrations of these particles in aqueous dispersions are in
the range of 1x1010 - 1x1011 particles per mL. We have bioconjugated these gold nanorods with anti-HER2/neu mouse
monoclonal antibodies (MAb). Since the as-prepared CTAB-stabilized nanorods were found to be toxic to SKBR3 cells,
we decided to coat the gold nanorods with polyethylene glycol (PEG). Characterization and size estimation of the
nanoparticles were performed using electron microscopies, optical spectroscopy and confocal microscopy. We present
these results and implications for use of these nanoparticles for in vivo biomedical applications.
Paper Details
Date Published: 11 July 2007
PDF: 10 pages
Proc. SPIE 6626, Molecular Imaging, 66260C (11 July 2007); doi: 10.1117/12.728226
Published in SPIE Proceedings Vol. 6626:
Molecular Imaging
Kai Licha; Vasilis Ntziachristos, Editor(s)
PDF: 10 pages
Proc. SPIE 6626, Molecular Imaging, 66260C (11 July 2007); doi: 10.1117/12.728226
Show Author Affiliations
R. G. Rayavarapu, Univ. of Twente (Netherlands)
W. Petersen, Univ. of Twente (Netherlands)
S. Le Gac, Univ. of Twente (Netherlands)
W. Petersen, Univ. of Twente (Netherlands)
S. Le Gac, Univ. of Twente (Netherlands)
C. Ungureanu, Univ. of Twente (Netherlands)
T. G. van Leeuwen, Univ. of Twente (Netherlands)
S. Manohar D.D.S., Univ. of Twente (Netherlands)
T. G. van Leeuwen, Univ. of Twente (Netherlands)
S. Manohar D.D.S., Univ. of Twente (Netherlands)
Published in SPIE Proceedings Vol. 6626:
Molecular Imaging
Kai Licha; Vasilis Ntziachristos, Editor(s)
© SPIE. Terms of Use
