
Proceedings Paper
Towards ultra-stable fluorescent dyes for single-molecule spectroscopyFormat | Member Price | Non-Member Price |
---|---|---|
$17.00 | $21.00 |
Paper Abstract
The wide-spread use of fluorescent dyes in molecular diagnostics and fluorescence microscopy together with new
developments such as single-molecule fluorescence spectroscopy provide researchers from various disciplines with an
ever expanding toolbox. Single-molecule fluorescence spectroscopy relies to a large extent on extraordinary bright and
photostable organic fluorescent dyes such as rhodamine- or cyanine- derivatives. While in the last decade singlemolecule
equipment and methodology have significantly advanced and in some cases reached theoretical limits (e.g.
detectors approaching unity quantum yields), instable emission ("blinking") and photobleaching become more and more
the bottleneck of further development and spreading of single-molecule fluorescence studies. In recent years, agents and
recipes have been developed to increase the photostability of conventional fluorescent dyes. Here, we investigate some
of these strategies at the single-molecule level. In particular, we focus on the dye selection criteria for multi-color
applications. We investigate fluorescent dyes from the rhodamine, carborhodamine, cyanine, and oxazine family and
show that within one dye class the photophysical properties are very similar but that dyes from different classes show
strikingly different properties. These findings facilitate dye selection and provide improved chemical environment for
demanding fluorescence microscopic applications.
Paper Details
Date Published: 13 July 2007
PDF: 12 pages
Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66331Z (13 July 2007); doi: 10.1117/12.728221
Published in SPIE Proceedings Vol. 6633:
Biophotonics 2007: Optics in Life Science
Jürgen Popp; Gert von Bally, Editor(s)
PDF: 12 pages
Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66331Z (13 July 2007); doi: 10.1117/12.728221
Show Author Affiliations
Robert Kasper, Univ. of Bielefeld (Germany)
Mike Heilemann, Univ. of Oxford (United Kingdom)
Mike Heilemann, Univ. of Oxford (United Kingdom)
Philip Tinnefeld, Univ. of Bielefeld (Germany)
Ludwig-Maximilians-Univ. (Germany)
Markus Sauer, Univ. of Bielefeld (Germany)
Ludwig-Maximilians-Univ. (Germany)
Markus Sauer, Univ. of Bielefeld (Germany)
Published in SPIE Proceedings Vol. 6633:
Biophotonics 2007: Optics in Life Science
Jürgen Popp; Gert von Bally, Editor(s)
© SPIE. Terms of Use
