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Proceedings Paper

Investigation of murine vasodynamics by Fourier domain optical coherence tomography
Author(s): Sven Meißner; Gregor Müller; Julia Walther; Alexander Krüger; Maximiliano Cuevas; Birgit Eichhorn; Ursula Ravens; Henning Morawietz; Edmund Koch
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Paper Abstract

In vivo imaging of blood vessels obtain useful insights in characterizing the dynamics of vasoconstriction and vasodilation. Fourier domain optical Coherence Tomography (FD-OCT) imaging technique permits in vivo investigation of blood vessels in their anatomical context without preparation traumata by temporal resolved image stacks. OCT is an optical, contact less imaging technique based on Michelson interferometry of short coherent near infrared light. Particularly by the possibility of a contact-less measurement and the high axial resolution up to 10 microns OCT is superior to an investigation by ultra sound measurement. Furthermore we obtain a high time resolution of vessel dynamic measurements with the used Fourier domain OCT-system by a high A-scan rate [1,22kHz]. In this study the model of saphenous artery was chosen for analyzing function and dynamics. The arteria saphena in the mouse is a suitable blood vessel due to the small inner diameter, a sensitive response to vasoactive stimuli and an advantageous anatomically position. Male wild type mice (C57BL/6) at the age of 8 weeks were fed control or high-fat diet for 10 weeks before analyzing the vasodynamics. The blood vessel was stimulated by dermal application of potassium to induce vasoconstriction or Sodium-Nitroprusside (SNP) to induce vasodilation. The morphology of the a. saphena and vein was determined by 3D image stacks. Time series (72 seconds, 300x512 pixel per frame) of cross-sectional images were analysed using semi automatic image processing software. Time course of dynamic parameters of the vessel was measured.

Paper Details

Date Published: 11 July 2007
PDF: 10 pages
Proc. SPIE 6627, Optical Coherence Tomography and Coherence Techniques III, 66270D (11 July 2007); doi: 10.1117/12.728163
Show Author Affiliations
Sven Meißner, Univ. of Technology Dresden (Germany)
Gregor Müller, Univ. of Technology Dresden (Germany)
Julia Walther, Univ. of Technology Dresden (Germany)
Alexander Krüger, Univ. of Technology Dresden (Germany)
Maximiliano Cuevas, Technische Univ. Dresden (Germany)
Birgit Eichhorn, Univ. of Technology Dresden (Germany)
Ursula Ravens, Technische Univ. Dresden (Germany)
Henning Morawietz, Univ. of Technology Dresden (Germany)
Edmund Koch, Univ. of Technology Dresden (Germany)

Published in SPIE Proceedings Vol. 6627:
Optical Coherence Tomography and Coherence Techniques III
Peter E. Andersen; Zhongping Chen, Editor(s)

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