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Proceedings Paper

SERS as analytical tool for detection of bacteria
Author(s): Dana Cialla; Petra Rösch; Robert Möller; Jürgen Popp
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Paper Abstract

The detection of single bacteria should be improved by lowering the acquisition time via the application of SERS (surface enhanced Raman spectroscopy). Nano structured colloids or surfaces consisting of gold or silver can be used as SERS active substrates. However, for biological applications mostly gold is used as SERS active substrate since silver is toxic for bacterial cells. Furthermore, the application of gold as a SERS-active substrate allows the usage of Raman excitation wavelengths in the red part of the electromagnetic spectrum. For the SERS investigations on bacteria different colloids (purchased and self prepared, preaggregated and non-aggregated) are chosen as SERS active substrates. The application of different gold colloids under gently mixing conditions to prevent the bacterial damage allowed the recording of reproducible SERS spectra of bacteria. The SERS spectra of B. pumilus are dominated by contributions of ingredients of the outer cell wall, e.g. the peptidoglycan layer. SEM images of the coated bacteria demonstrate the incomplete adsorption most probably due to variations within the binding affinities between different outer cell components and the gold colloids.

Paper Details

Date Published: 13 July 2007
PDF: 10 pages
Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66331U (13 July 2007);
Show Author Affiliations
Dana Cialla, Friedrich-Schiller-Univ. Jena (Germany)
Petra Rösch, Friedrich-Schiller-Univ. Jena (Germany)
Robert Möller, Friedrich-Schiller-Univ. Jena (Germany)
Jürgen Popp, Friedrich-Schiller-Univ. Jena (Germany)
Institute of Photonic Technology Jena (Germany)

Published in SPIE Proceedings Vol. 6633:
Biophotonics 2007: Optics in Life Science
Jürgen Popp; Gert von Bally, Editor(s)

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