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Proceedings Paper

Utilizing nonlinear optical microscopy to investigate the development of early cancer in nude mice in vivo
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Paper Abstract

In this investigation, we used in vivo nonlinear optical microscopy to image normal and carcinogen DMBA treated skin tissues of nude mice. We acquired two-photon autofluroescence and second harmonic generation (SHG) images of the skin tissue, and applied the ASI (Autofluorescence versus SHG Index) to the resulting image. This allows us to visualize and quantify the interaction between mouse skin cells and the surrounding connective tissue. We found that as the imaging depth increases, ASI has a different distribution in the normal and the treated skin tissues. Since the DMBA treated skin eventually became squamous cell carcinoma (SCC), our results show that the physiological changes to mouse skin en route to become cancer can be effectively tracked by multiphoton microscopy. We envision this approach to be effective in studying tumor biology and tumor treatment procedures.

Paper Details

Date Published: 20 July 2007
PDF: 8 pages
Proc. SPIE 6630, Confocal, Multiphoton, and Nonlinear Microscopic Imaging III, 66300Y (20 July 2007); doi: 10.1117/12.728066
Show Author Affiliations
Chun-Chin Wang, National Taiwan Univ. (Taiwan)
Feng-Chieh Li, National Taiwan Univ. (Taiwan)
Sung-Jan Lin, National Taiwan Univ. Hospital and College of Medicine (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6630:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging III
Tony Wilson; Ammasi Periasamy, Editor(s)

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