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Proceedings Paper

Optical switches: key molecules for improved fluorescence imaging and tracking with high optical resolution
Author(s): Philip Tinnefeld; Jochen Mattay; Karl Heinz Drexhage; Markus Sauer
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Paper Abstract

An optical switch exhibits two stable and selectively addressable states, a fluorescent state and a non-fluorescent state, which can be reversibly interconverted upon irradiation with different wavelengths of light. Efficient molecular optical switches are strongly desired for improved protein tracking in living cells and they are potentially very useful for far-field fluorescence imaging with improved resolution. Because suitable optical switches reversibly undergo light-induced transitions between two thermally stable states, and the transitions are saturable, a spatial intensity distribution of two laser wavelengths (one to switch off the fluorophore and another to reactivate fluorescence) featuring a local minimum might allow fluorescence imaging at the nanoscale. Conceptually similar to stimulated-emission depletion (STED) microscopy optical switches promise similar spatial resolution, but require much lower laser intensities. Here we review different concepts that use reversible saturable optical fluorescence transitions (RESOLFT) and discuss the requirements optical switches have to fulfill. In addition, we address the problem of selective in vivo labeling of target molecules with chemical switches, i.e. optical switches that are activated upon specific labeling to target molecules in living cells.

Paper Details

Date Published: 13 July 2007
PDF: 10 pages
Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 663303 (13 July 2007); doi: 10.1117/12.728016
Show Author Affiliations
Philip Tinnefeld, Ludwig-Maximilians-Univ. (Germany)
Bielefeld Univ. (Germany)
Jochen Mattay, Bielefeld Univ. (Germany)
Karl Heinz Drexhage, Univ. of Siegen (Germany)
Markus Sauer, Bielefeld Univ. (Germany)

Published in SPIE Proceedings Vol. 6633:
Biophotonics 2007: Optics in Life Science
Jürgen Popp; Gert von Bally, Editor(s)

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