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Proceedings Paper

Three-dimensional Fourier-domain optical coherence tomography of alveolar mechanics in stepwise inflated and deflated isolated and perfused rabbit lungs
Author(s): Alexander Krueger; Lilla Knels; Sven Meissner; Martina Wendel; Axel R. Heller; Thomas Lambeck; Thea Koch; Edmund Koch
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Paper Abstract

Fourier domain optical coherence tomography (FD-OCT) was used to acquire three-dimensional image stacks of isolated and perfused rabbit lungs (n = 4) at different constant pulmonary airway pressures (CPAP) and during vascular fixation. After despeckling and applying a threshold, the images were segmented into air and tissue, and registered to each other to compensate for movement between CPAP steps. The air-filled cross-sectional areas were quantified using a semi-automatic algorithm. The cross-sectional area of alveolar structures taken at all three perpendicular planes increased with increasing CPAP. Between the minimal CPAP of 3 mbar and the maximum of 25 mbar the areas increased to about 140% of their initial value. There was no systematic dependency of inflation rate on initial size of the alveolar structure. During the perfusion fixation of the lungs with glutaraldehyde morphometric changes of the alveolar geometry measured with FD-OCT were negligible.

Paper Details

Date Published: 11 July 2007
PDF: 10 pages
Proc. SPIE 6627, Optical Coherence Tomography and Coherence Techniques III, 662707 (11 July 2007); doi: 10.1117/12.727891
Show Author Affiliations
Alexander Krueger, Technische Univ. Dresden (Germany)
Lilla Knels, Technische Univ. Dresden (Germany)
Sven Meissner, Technische Univ. Dresden (Germany)
Martina Wendel, Technische Univ. Dresden (Germany)
Axel R. Heller, Technische Univ. Dresden (Germany)
Thomas Lambeck, Technische Univ. Dresden (Germany)
Thea Koch, Technische Univ. Dresden (Germany)
Edmund Koch, Technische Univ. Dresden (Germany)

Published in SPIE Proceedings Vol. 6627:
Optical Coherence Tomography and Coherence Techniques III
Peter E. Andersen; Zhongping Chen, Editor(s)

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