
Proceedings Paper
In vivo micro-lesion of single dendrite with femtosecond laser pulsesFormat | Member Price | Non-Member Price |
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Paper Abstract
Recently, two-photon microscopy has been used for high spatial resolution imaging of the intact neocortex in living
rodents. In this work we used near-IR femtosecond laser pulses for a combination of two-photon microscopy and
microdissection on fluorescently-labeled neuronal structures in living mice. Three-dimensional reconstructions of
dendrites expressing the green fluorescence protein were made in the cortex of GFP-M and YFP-H transgenic mice.
Afterwards, single dendrites were laser-dissected irradiating the structure with a high femtosecond laser energy dose. We
report that laser dissection can be performed with micrometric precision and without any visible collateral damage of the
surrounding neuronal structures. After laser irradiation, one part of the severed dendrite underwent degeneration and
disappeared within 5 hours.
Using a chronically implanted glass window, we performed long-term imaging in the area of the dissected dendrite.
Images of the long-term morphological changes in the neuronal network after dendritic lesioning will be provided. Laser
microdissection of selected structures of the neuronal branching in vivo represents a promising tool for neurobiological
research.
Paper Details
Date Published: 13 July 2007
PDF: 7 pages
Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 663310 (13 July 2007); doi: 10.1117/12.727874
Published in SPIE Proceedings Vol. 6633:
Biophotonics 2007: Optics in Life Science
Jürgen Popp; Gert von Bally, Editor(s)
PDF: 7 pages
Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 663310 (13 July 2007); doi: 10.1117/12.727874
Show Author Affiliations
L. Sacconi, L.E.N.S., Univ. of Florence (Italy)
A. Masi, L.E.N.S., Univ. of Florence (Italy)
Univ. of Florence (Italy)
G. Diana, Istituto Superiore di Sanità (Italy)
A. Masi, L.E.N.S., Univ. of Florence (Italy)
Univ. of Florence (Italy)
G. Diana, Istituto Superiore di Sanità (Italy)
M. Buffelli, Univ. of Verona (Italy)
F. S. Pavone, L.E.N.S., Univ. of Florence (Italy)
Univ. of Florence (Italy)
F. S. Pavone, L.E.N.S., Univ. of Florence (Italy)
Univ. of Florence (Italy)
Published in SPIE Proceedings Vol. 6633:
Biophotonics 2007: Optics in Life Science
Jürgen Popp; Gert von Bally, Editor(s)
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