Proceedings Paper
Efficacy of a single high dose versus multiple low doses of LLLT on wounded skin fibroblasts| Format | Member Price | Non-Member Price |
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Paper Abstract
Background/purpose: In vivo studies have demonstrated that phototherapy accelerates wound healing in the clinical
environment; however the exact mechanism is still not completely understood. The main focus of this study was to use
in vitro laboratory results to establish an effective treatment regimen that may be practical and applicable to the clinical
environment. This in vitro study aimed to compare the cellular responses of wounded fibroblasts following a single
exposure of 5 J/cm2 or multiple exposures of low doses (2.5 J/cm2 or 5 J/cm2) on one day of the week to a single
application of a higher dose (16 J/cm2) on day 1 and day 4. Methodology: Cellular responses to Helium-Neon
(632.8 nm) laser irradiation were evaluated by measuring changes in cell morphology, cell viability, cell proliferation,
membrane integrity and DNA damage. Results: Wounded cells exposed to 5 J/cm2 on day 1 and day 4 showed an
increase in cell viability, increase in the release of bFGF, increase in cell density, decrease in ALP enzyme activity and
decrease in caspase 3/7 activity indicating a stimulatory effect. Wounded cells exposed to three doses of 5 J/cm2 on day
1 showed a decrease in cell viability and cell proliferation and an increase in LDH cytotoxicity and DNA damage
indicating an inhibitory effect. Conclusion: Results indicate that cellular responses are influenced by the combination of
dose administered, number of exposures and time between exposures. Single doses administered with sufficient time
between exposures is more beneficial to restoring cell function than multiple doses within a short period. Although this
work confirms previous reports on the cumulative effect of laser irradiation it provides essential information for the
initiation of in vivo clinical studies.
Paper Details
Date Published: 13 July 2007
PDF: 12 pages
Proc. SPIE 6632, Therapeutic Laser Applications and Laser-Tissue Interactions III, 66321U (13 July 2007); doi: 10.1117/12.725409
Published in SPIE Proceedings Vol. 6632:
Therapeutic Laser Applications and Laser-Tissue Interactions III
Alfred Vogel, Editor(s)
PDF: 12 pages
Proc. SPIE 6632, Therapeutic Laser Applications and Laser-Tissue Interactions III, 66321U (13 July 2007); doi: 10.1117/12.725409
Show Author Affiliations
Denise H. Hawkins, Univ. of Johannesburg (South Africa)
Heidi Abrahamse, Univ. of Johannesburg (South Africa)
Published in SPIE Proceedings Vol. 6632:
Therapeutic Laser Applications and Laser-Tissue Interactions III
Alfred Vogel, Editor(s)
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