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Proceedings Paper

The MicroActive project: automatic detection of disease-related molecular cell activity
Author(s): Liv Furuberg; Michal Mielnik; Ib-Rune Johansen; Jörg Voitel; Anja Gulliksen; Lars Solli; Frank Karlsen; Tobias Bayer; Friedhelm Schönfeld; Klaus Drese; Helen Keegan; Cara Martin; John O'Leary; Lutz Riegger; Peter Koltay
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Paper Abstract

The aim of the MicroActive project is to develop an instrument for molecular diagnostics. The instrument will first be tested for patient screening for a group of viruses causing cervical cancer. Two disposable polymer chips with reagents stored on-chip will be inserted into the instrument for each patient sample. The first chip performs sample preparation of the epithelial cervical cells while mRNA amplification and fluorescent detection takes place in the second chip. More than 10 different virus markers will be analysed in one chip. We report results on sub-functions of the amplification chip. The sample is split into smaller droplets, and the droplets move in parallel channels containing different dried reagents for the different analyses. We report experimental results on parallel droplet movement control using one external pump only, combined with hydrophobic valves. Valve burst pressures are controlled by geometry. We show droplet control using valves with burst pressures between 800 and 4500 Pa. We also monitored the re-hydration times for two necessary dried reagents. After sample insertion, uniform concentration of the reagents in the droplet was reached after respectively 60 s and 10 min. These times are acceptable for successful amplification. Finally we have shown positive amplification of HPV type 16 using dried enzymes stored in micro chambers.

Paper Details

Date Published: 15 May 2007
PDF: 12 pages
Proc. SPIE 6589, Smart Sensors, Actuators, and MEMS III, 65891B (15 May 2007); doi: 10.1117/12.724785
Show Author Affiliations
Liv Furuberg, SINTEF Microsystems and Nanotechnology (Norway)
Michal Mielnik, SINTEF Microsystems and Nanotechnology (Norway)
Ib-Rune Johansen, SINTEF Microsystems and Nanotechnology (Norway)
Jörg Voitel, SINTEF Microsystems and Nanotechnology (Norway)
Anja Gulliksen, Norchip AS (Norway)
Univ. of Oslo (Norway)
Lars Solli, Norchip AS (Norway)
NTNU (Norway)
Frank Karlsen, Norchip AS (Norway)
Tobias Bayer, Institut für Mikrotechnik Mainz GmbH (Germany)
Friedhelm Schönfeld, Institut für Mikrotechnik Mainz GmbH (Germany)
Klaus Drese, Institut für Mikrotechnik Mainz GmbH (Germany)
Helen Keegan, Coombe Women's Hospital (Ireland)
IMTEK (Germany)
Cara Martin, Coombe Women's Hospital (Ireland)
IMTEK (Germany)
John O'Leary, Coombe Women's Hospital (Ireland)
IMTEK (Germany)
Lutz Riegger, IMTEK (Germany)
Peter Koltay, BioFluidix (Germany)

Published in SPIE Proceedings Vol. 6589:
Smart Sensors, Actuators, and MEMS III
Thomas Becker; Carles Cané; N. Scott Barker, Editor(s)

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