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Proceedings Paper

Toward new fluorescent bioinspired sensors: interaction of poly(fluorene-phenylene) with phospholipid bilayers
Author(s): R. Mallavia; F. J. Paya; A. Salinas; A. Estepa; C. R. Mateo
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Paper Abstract

Most of the conjugated polymer employed as fluorescent biosensors present low solubility and emission in aqueous environment. In order to solve this feature, we have reconstituted, in buffer phosphate, a neutral conjugated poly[9,9- bis(6'-bromohexil)-2,7-fluorene-co-alt-1,4-phenylene], as PFPBr2 (insoluble in water), in the presence of an artificial zwitterionic phospholipids bilayers, as 1,2-dimyristoyl-sn-glycero-3-phospho-choline (DMPC). Quantum yield of PFPBr2-DMPC was around 20% in phosphate buffer, it was identical value calculated from ammonium polyelectrolytes (PFPNMe3+). In addition, the maximum of bluish emission for buffer solution of PFPBr2-DMPC was at 420nm, a red-shift emission with regard to chloroform solution (at 410 nm). The structural study at different concentrations of PFPBr2 and DMPC was carried out using different approaches: steady state fluorescence spectroscopy, confocal fluorescence microscopy and calorimetry. A positive interaction takes place involving neutral conjugated polymer and zwitterionic phospholipids bilayer. Novels complexes or associations of poly(fluorene-phenylene) (PFPBr2) and zwitterionic phospholipids (DMPC) have been suggested and visualized by epifluorescence. Phase transitions of the liposomes have been also detected by differential scanning calorimetry.

Paper Details

Date Published: 22 May 2007
PDF: 8 pages
Proc. SPIE 6592, Bioengineered and Bioinspired Systems III, 659213 (22 May 2007); doi: 10.1117/12.721505
Show Author Affiliations
R. Mallavia, Univ. Miguel Hernández de Elche (Spain)
F. J. Paya, Univ. Miguel Hernández de Elche (Spain)
A. Salinas, Univ. Miguel Hernández de Elche (Spain)
A. Estepa, Univ. Miguel Hernández de Elche (Spain)
C. R. Mateo, Univ. Miguel Hernández de Elche (Spain)

Published in SPIE Proceedings Vol. 6592:
Bioengineered and Bioinspired Systems III
Paolo Arena; Ángel Rodríguez-Vázquez; Gustavo Liñán-Cembrano, Editor(s)

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