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Proceedings Paper

Track and trap in 3D
Author(s): Jesper Glückstad; Peter J. Rodrigo; Ivan P. Nielsen; Carlo A. Alonzo
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Paper Abstract

Three-dimensional light structures can be created by modulating the spatial phase and polarization properties of an an expanded laser beam. A particularly promising technique is the Generalized Phase Contrast (GPC) method invented and patented at Risø National Laboratory. Based on the combination of programmable spatial light modulator devices and an advanced graphical user-interface the GPC method enables real-time, interactive and arbitrary control over the dynamics and geometry of synthesized light patterns. Recent experiments have shown that GPC-driven micro-manipulation provides a unique technology platform for fully user-guided assembly of a plurality of particles in a plane, control of particle stacking along the beam axis, manipulation of multiple hollow beads, and the organization of living cells into three-dimensional colloidal structures. Here we present GPC-based optical micromanipulation in a microfluidic system where trapping experiments are computer-automated and thereby capable of running with only limited supervision. The system is able to dynamically detect living yeast cells using a computer-interfaced CCD camera, and respond to this by instantly creating traps at positions of the spotted cells streaming at flow velocities that would be difficult for a human operator to handle.

Paper Details

Date Published: 9 April 2007
PDF: 9 pages
Proc. SPIE 6574, Optical Pattern Recognition XVIII, 657403 (9 April 2007); doi: 10.1117/12.719186
Show Author Affiliations
Jesper Glückstad, Risø National Lab. (Denmark)
Peter J. Rodrigo, Risø National Lab. (Denmark)
Ivan P. Nielsen, Risø National Lab. (Denmark)
Carlo A. Alonzo, Risø National Lab. (Denmark)

Published in SPIE Proceedings Vol. 6574:
Optical Pattern Recognition XVIII
David P. Casasent; Tien-Hsin Chao, Editor(s)

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