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Proceedings Paper

New components for fiber-optic thin-layer chromatography including fluorescence
Author(s): Michaela Bleichert; Hanns Simon Eckhardt; Karl-Friedrich Klein; Bernd Spangenberg; Georg Hillrichs; Joachim Mannhardt
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Paper Abstract

In thin-layer chromatography, fiber-bundle arrays have been introduced for spectral absorption measurements in the UV-region. Using all-silica fiber bundles, the exciting light will be detected after re-emission on the plate with a fiberoptic spectrometer. In addition, fluorescence light can be detected which will be masked by the re-emitted light. Therefore, it is helpful to separate the absorption and fluorescence on the TLC-plate. A modified three-array assembly has been developed: using one array for detection, the two others are used for excitation with broadband band deuterium-light and with UV-LEDs adjusted to the substances under test. As an example, the quantification of glucosamine in nutritional supplements or spinach leaf extract will be described. Using simply heating of the amino-plate for derivation, the reaction product of Glucosamine can be detected sensitively either by light absorption or by fluorescence, using the new fiber-optic assembly. In addition, the properties of the new 3-row fiber-optic array and the commercially available UV-LEDs will be shown, in the interesting wavelength region for excitation of fluorescence, from 260 nm to 360 nm. The squint angle having an influence on coupling efficiency and spatial resolution will be measured with the inverse farfield method. Some properties of UV-LEDs for analytical applications will be described and discussed, too.

Paper Details

Date Published: 15 February 2007
PDF: 10 pages
Proc. SPIE 6433, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII, 64330F (15 February 2007); doi: 10.1117/12.717579
Show Author Affiliations
Michaela Bleichert, Univ. of Applied Sciences Offenburg (Germany)
Hanns Simon Eckhardt, Univ. of Applied Sciences Giessen Friedberg (Germany)
Karl-Friedrich Klein, Univ. of Applied Sciences Giessen Friedberg (Germany)
Bernd Spangenberg, Univ. of Applied Sciences Offenburg (Germany)
Georg Hillrichs, Univ. of Applied Sciences Merseburg (Germany)
Joachim Mannhardt, J&M Analytische Mess- und Regeltechnik GmbH (Germany)

Published in SPIE Proceedings Vol. 6433:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII
Israel Gannot, Editor(s)

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