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Proceedings Paper

Single quantum dot fluorescence resonant energy transfer: probing the heterogeneity in macroscopic samples
Author(s): Thomas Pons; Igor L. Medintz; Xiang Wang; Douglas S. English; Hedi Mattoussi
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Paper Abstract

We present a single QD FRET study that allows us to probe the heterogeneity in QD-dye labeled protein conjugates. We first show that QDs are compatible with spFRET detection by demonstrating the equivalence of single particle and ensemble measurement modalities in terms of derived average FRET efficiencies and separation distances between a QD donor and dyes attached to specific sites on conjugated proteins. We then use spFRET data to demonstrate that the valence distribution of QD-protein conjugates follows Poisson statistics.

Paper Details

Date Published: 27 February 2007
PDF: 9 pages
Proc. SPIE 6448, Colloidal Quantum Dots for Biomedical Applications II, 64480I (27 February 2007); doi: 10.1117/12.717539
Show Author Affiliations
Thomas Pons, Naval Research Lab. (United States)
Igor L. Medintz, Naval Research Lab. (United States)
Xiang Wang, Univ. of Maryland, College Park (United States)
Douglas S. English, Univ. of Maryland, College Park (United States)
Hedi Mattoussi, Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 6448:
Colloidal Quantum Dots for Biomedical Applications II
Marek Osinski; Thomas M. Jovin; Kenji Yamamoto, Editor(s)

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