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Proceedings Paper

Plasmon-resonant nanorods as multimodal agents for two-photon luminescence imaging and photothermal therapy
Author(s): Terry B. Huff; Matthew N. Hansen; Ling Tong; Yan Zhao; Haifeng Wang; Daniel A. Zweifel; Ji-Xin Cheng; Alexander Wei
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Paper Abstract

Plasmon-resonant gold nanorods have outstanding potential as multifunctional agents for image-guided therapies. Nanorods have large absorption cross sections at near-infrared (NIR) frequencies, and produce two-photon luminescence (TPL) when excited by fs-pulsed laser irradiation. The TPL signals can be detected with single-particle sensitivity, enabling nanorods to be imaged in vivo while passing through blood vessels at subpicomolar concentrations. Furthermore, cells labeled with nanorods become highly susceptible to photothermal damage when irradiated at plasmon resonance, often resulting in a dramatic blebbing of the cell membrane. However, the straightforward application of gold nanorods for cell-specific labeling is obstructed by the presence of CTAB, a cationic surfactant carried over from nanorod synthesis which also promotes their nonspecific uptake into cells. Careful exchange and replacement of CTAB can be achieved by introducing oligoethyleneglycol (OEG) units capable of chemisorption onto nanorod surfaces by in situ dithiocarbamate formation, a novel method of surface functionalization. Nanorods with a dense coating of methyl-terminated OEG chains are shielded from nonspecific cell uptake, whereas nanorods functionalized with folate-terminated OEG chains accumulate on the surface of tumor cells overexpressing their cognate receptor, with subsequent delivery of photoinduced cell damage at low laser fluence.

Paper Details

Date Published: 5 February 2007
PDF: 9 pages
Proc. SPIE 6448, Colloidal Quantum Dots for Biomedical Applications II, 64480D (5 February 2007); doi: 10.1117/12.717537
Show Author Affiliations
Terry B. Huff, Purdue Univ. (United States)
Matthew N. Hansen, Purdue Univ. (United States)
Ling Tong, Purdue Univ. (United States)
Yan Zhao, Purdue Univ. (United States)
Haifeng Wang, Purdue Univ. (United States)
Daniel A. Zweifel, Purdue Univ. (United States)
Ji-Xin Cheng, Purdue Univ. (United States)
Alexander Wei, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 6448:
Colloidal Quantum Dots for Biomedical Applications II
Marek Osinski; Thomas M. Jovin; Kenji Yamamoto, Editor(s)

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