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Proceedings Paper

Recording the diffraction pattern reflected from corneal endothelium
Author(s): Curry Bucht; Per Söderberg; Göran Manneberg
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Paper Abstract

As a part of an ongoing research project on morphometrical diagnosis of the corneal endothelium, an experimental optical setup has been created. The structure of the corneal endothelial cells could be considered a reflecting periodical aperture. Hence, the diffraction pattern reflected from the endothelium contains valuable morphometrical information. In the present work, focus has been on sampling the posterior surface of explanted corneas. Methods: An optical setup was created, using a 632.8 nm He-Ne laser as the light source. The desired diffraction pattern was produced as a collimated reflection. Hence, because the posterior surface of the cornea is concave, lenses were used to attain the right divergence of the light impingent on the corneal endothelium. These lenses also made it possible to adjust the sampling spot size. A beam splitter (BS) was used to provide an optical path for both the impinging laser beam as well as the reflected diffracted beam. The lens acting as a Fourier lens was then placed after the BS. At the back focal plane of the Fourier lens, a CCD detector was used for recording in the Fourier plane. In the process of creating the setup, explanted corneas were emulated using grated contact lenses. Results: The current optical set up allows identification of a diffraction pattern from a concave spherical surface with a radius of curvature of the same order as a human cornea.

Paper Details

Date Published: 5 March 2007
PDF: 8 pages
Proc. SPIE 6426, Ophthalmic Technologies XVII, 642610 (5 March 2007); doi: 10.1117/12.717478
Show Author Affiliations
Curry Bucht, St Erik's Eye Hospital (Sweden)
Stockholm's Royal Institute of Technology (Sweden)
Per Söderberg, St Erik's Eye Hospital (Sweden)
Univ. of Miami (United States)
Göran Manneberg, Stockholm's Royal Institute of Technology (Sweden)

Published in SPIE Proceedings Vol. 6426:
Ophthalmic Technologies XVII
Bruce E. Stuck; Fabrice Manns; Per G. Söderberg; Michael Belkin M.D.; Arthur Ho, Editor(s)

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