
Proceedings Paper
PDMS microdevice with built-in optical biosensor array for on-site monitoring of the microenvironment within microchannelsFormat | Member Price | Non-Member Price |
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Paper Abstract
Nowadays as cell culture in microchannels becomes increasingly popular, new and convenient ways to closely monitor
the cultured cells in the microchannels are essential. With that objective, we managed to merge different technologies,
mainly fluorescence based optical sensing, layer-by-layer encapsulation and Poly Dimethyl Siloxane (PDMS) based
microfluidics to build a hybrid system which can provide on-site monitoring of the cellular microenvironment within the
microchannels. The system is mainly built from two parts: one is the fluorescence quenching based sensing material
which changes its fluorescence intensity proportional to the analyte concentration, for example glucose level in the
media; the other part of the system is the microfluidic PDMS chip, featuring some nanoliter wells fabricated beneath the
microchannels where the cells could be cultured. These nanowells are intended sites for the immobilization of the
sensing material followed by the layer-by-layer encapsulation. Some preliminary results show that the system is
sensitive in low analyte concentration, while being highly miniaturized, low cost, easy to be customized and with great
potential applications in medical diagnostics and environmental monitoring.
Paper Details
Date Published: 15 February 2007
PDF: 8 pages
Proc. SPIE 6445, Optical Diagnostics and Sensing VII, 64450W (15 February 2007); doi: 10.1117/12.716250
Published in SPIE Proceedings Vol. 6445:
Optical Diagnostics and Sensing VII
Gerard L. Coté; Alexander V. Priezzhev, Editor(s)
PDF: 8 pages
Proc. SPIE 6445, Optical Diagnostics and Sensing VII, 64450W (15 February 2007); doi: 10.1117/12.716250
Show Author Affiliations
Lin Zhang, National Univ. of Singapore (Singapore)
Kwan Yee Cheung, National Univ. of Singapore (Singapore)
Kwan Yee Cheung, National Univ. of Singapore (Singapore)
Ya Ru Chua, National Univ. of Singapore (Singapore)
Dieter Trau, National Univ. of Singapore (Singapore)
Dieter Trau, National Univ. of Singapore (Singapore)
Published in SPIE Proceedings Vol. 6445:
Optical Diagnostics and Sensing VII
Gerard L. Coté; Alexander V. Priezzhev, Editor(s)
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