The easy and non-destructive fluorescence method for quantification of early changes in biological tissues improves the possibilities of the clinical research. Using endogenous and exogenous fluorophores one can achieve high accuracy in the determination of malignant melanoma lesions with wide clinical applications. The goals of this work were investigation of melanin-pigmented cutaneous lesions by the methods of laser-induced autofluorescence spectroscopy (LIAFS), using nitrogen laser (337 nm) as excitation source. All lesions investigated were excised and investigated histologically by standard methods and histological results were used for comparison with the spectra detected. In the cases of nevi and melanoma significant decrease of fluorescence intensity toward normal skin fluorescence, which correlated with the type of pigment lesion was observed. The results, obtained in this investigation of the different pigment lesions could be used for better comprehension of the skin optical properties. The fluorescence spectroscopy of the human skin is very prominent for early diagnosis and differentiation of cutaneous diseases.

Date Published: 12 September 2006
PDF: 8 pages
Proc. SPIE 6284, International Conference on Lasers, Applications, and Technologies 2005: Laser Technologies for Environmental Monitoring and Ecological Applications, and Laser Technologies for Medicine, 62840K (12 September 2006); doi: 10.1117/12.714174

Published in SPIE Proceedings Vol. 6284:
International Conference on Lasers, Applications, and Technologies 2005: Laser Technologies for Environmental Monitoring and Ecological Applications, and Laser Technologies for Medicine
Martin Frenz; Andrey V. Larichev; Gennadii G. Matvienko; Vladislav Ya. Panchenko; Upendra N. Singh, Editor(s)