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Proceedings Paper

Compound imaging of ocular tissues with backward second harmonic generation and two-photon excitation fluorescence
Author(s): Danni Chen; Junle Qu; Gaixia Xu; Lixin Liu; Ziyang Lin; Hanben Niu
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Paper Abstract

We report the implementation of compound imaging with backward second harnionic generation (SHG) and two-photon excitation fluorescence (TPEF) in a standard confocal microscope. Various biological tissues, including skin, muscle and ocular tissues (e.g. cornea. iris, choroid, and sclera) have been investigated with different sample preparation methods (fresh, fixation). The results show that collagen-rich ocular tissues, like cornea, iris, choroids, and sclera can produce strong SHG signals. In sclera, there are not only plenty of collagen, but also various endogenous fluorophores. The organization of collagen fibers in sclera is clearly distinguishable from its SHG images viewed transversely and horizontally respectively. We also find that fixation of the sample with formaldehyde has reduced the intensity of SHG intensity by almost 5O%. As the SHG and TPEF can provide complementary information about collagen and fluorophores rich biological tissues, compound imaging of SHG and TPEF presented in this study is believed to have potential applications in biomedicine and clinical diagnosis.

Paper Details

Date Published: 27 October 2006
PDF: 7 pages
Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 604718 (27 October 2006); doi: 10.1117/12.710887
Show Author Affiliations
Danni Chen, Shenzhen Univ. (China)
Junle Qu, Shenzhen Univ. (China)
Gaixia Xu, Shenzhen Univ. (China)
Lixin Liu, Shenzhen Univ. (China)
Ziyang Lin, Shenzhen Univ. (China)
Hanben Niu, Shenzhen Univ. (China)

Published in SPIE Proceedings Vol. 6047:
Fourth International Conference on Photonics and Imaging in Biology and Medicine
Kexin Xu; Qingming Luo; Da Xing; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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