
Proceedings Paper
Analysis of biomodulative effects of low intensity laser on human skin fibroblast cells using fiber-optic nano-probesFormat | Member Price | Non-Member Price |
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Paper Abstract
Over the past few decades, many efforts were devoted to study low power laser and cellular interaction. Some of the
investigations were performed on cell populations. In this work fiber-optic based nano-probe is used for the precise
delivery of laser light on to a single cell and the mechanism of light interaction with the cell during irradiation was
studied. A human skin fibroblast cell line was utilized in this investigation. The human fibroblasts were irradiated under
two different schemes of exposure: (1) entire cell population was irradiated within a Petri dish using a fan beam, (2)
laser energy was precisely delivered on to a single cell using fiber-optic nano-probe. Studies were conducted by
variation of laser intensity, exposure time, and the energy dose of exposure. Proliferative effect of laser irradiation was
determined through cell counting for both exposure schemes. Enhancement of the rate of proliferation was observed to
be dependent on laser parameters and method of laser delivery. Variation of total energy dose had greater effect on the
enhancement of the rate of cellular proliferation compared to that of laser intensity. The photobiostimulative effect was
also observed to have a finite life-time. Fluorescent life-time imaging of reactive oxygen species (ROS) was performed
during the single cell exposure method. ROS generation was found to depend strongly on both laser energy doses and
irradiation time. It is demonstrated in this communication that by using specially engineered nano-probes, laser light can
be precisely delivered on to a targeted single cell.
Paper Details
Date Published: 21 February 2007
PDF: 10 pages
Proc. SPIE 6428, Mechanisms for Low-Light Therapy II, 64280D (21 February 2007); doi: 10.1117/12.710722
Published in SPIE Proceedings Vol. 6428:
Mechanisms for Low-Light Therapy II
Michael R. Hamblin; Ronald W. Waynant; Juanita Anders, Editor(s)
PDF: 10 pages
Proc. SPIE 6428, Mechanisms for Low-Light Therapy II, 64280D (21 February 2007); doi: 10.1117/12.710722
Show Author Affiliations
Ashim Dutta, Florida Institute of Technology (United States)
Kunal Mitra, Florida Institute of Technology (United States)
Michael S. Grace, Florida Institute of Technology (United States)
Ronald W. Waynant, U.S. Food and Drug Administration (United States)
Kunal Mitra, Florida Institute of Technology (United States)
Michael S. Grace, Florida Institute of Technology (United States)
Ronald W. Waynant, U.S. Food and Drug Administration (United States)
Darrell B. Tata, U.S. Food and Drug Administration (United States)
Eric Gorman, U.S. Food and Drug Administration (United States)
Juanita Anders, Uniformed Services Univ. of the Health Sciences (United States)
Eric Gorman, U.S. Food and Drug Administration (United States)
Juanita Anders, Uniformed Services Univ. of the Health Sciences (United States)
Published in SPIE Proceedings Vol. 6428:
Mechanisms for Low-Light Therapy II
Michael R. Hamblin; Ronald W. Waynant; Juanita Anders, Editor(s)
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