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Proceedings Paper

Multifocal two-photon excitation fluorescence sampling imaging combining lifetime and spectrum resolutions
Author(s): Lixin Liu; Ziyang Lin; Junle Qu; Danni Chen; Gaixia Xu; Baoping Guo; Hanben Niu
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Paper Abstract

Multifocal multiphoton microscopy (MMM) is a more efficient and powerful method for three-dimensional (3-D) fluorescence imaging with reduced acquisition time compared with conventional confocal and two-photon excitation fluorescence microscopy. We present a novel multifocal two-photon excitation fluorescence sampling imaging technique that is based on a specially designed streak camera and combines fluorescence lifetime and spectrum resolutions. A proof-of-principle experiment is performed on a standard fluorescent dye solution (Rhodamine 6G in ethanol), Time- and spectrum-resolved sampled fluorescence image of Rhodamine 6G is obtained in a snapshot. The reconstructed two-dimensional (2-D) fluorescence image of a prepared plant slide is also obtained by moving the sample laterally. The capability of this system capable of performing simultaneous 2-D measurements of temporal and spectral information has many potential applications, e.g., multi-well imaging and spectrally resolved multifocal multiphoton fluorescence lifetime imaging etc.

Paper Details

Date Published: 27 October 2006
PDF: 6 pages
Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60472T (27 October 2006); doi: 10.1117/12.710079
Show Author Affiliations
Lixin Liu, Xi'an Institute of Optics and Precision Mechanics (China)
Shenzhen Univ. (China)
Ziyang Lin, Shenzhen Univ. (China)
Junle Qu, Shenzhen Univ. (China)
Danni Chen, Shenzhen Univ. (China)
Gaixia Xu, Shenzhen Univ. (China)
Baoping Guo, Shenzhen Univ. (China)
Hanben Niu, Shenzhen Univ. (China)

Published in SPIE Proceedings Vol. 6047:
Fourth International Conference on Photonics and Imaging in Biology and Medicine
Kexin Xu; Qingming Luo; Da Xing; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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