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Proceedings Paper

How could the light fluence rate influence the tumor cell-killing rate in photodynamic therapy?
Author(s): Mingzhao Lee; Tao Xu
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Paper Abstract

Some work had been done in this paper to study the role of the fluence rate of the illuminating light in the photodynamic therapy (PDT), which focused on the influence of light fluence rate on the microvasculature damage, the cell killing and the photodynamic reaction impetus. The microvasculature damage was studied through observing the values of RBC's column diameter during the process of the HpD mediated PDT. It was found that less microvasculature damage was induced by 75 mW/cm2 illumination than that by 150 mW/cm2, indicating that under 75mW/cm2 illumination tumor oxygen can be better preserved than 150 mW/cm2. The cell killing experiment was performed in vitro and designed in the manner that cell killing rate was only influenced by photobleaching. Higher cell killing rate caused by 75 mW/cm illumination indicated that lower fluence rate light could enhance the light absorbency and decrease the bleaching of photosensitizer. So the cell-killing rate under low fluence rate was enhanced through the oxygen preservation and photobleaching decreasing.

Paper Details

Date Published: 27 October 2006
PDF: 6 pages
Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60470D (27 October 2006); doi: 10.1117/12.709844
Show Author Affiliations
Mingzhao Lee, Shenzhen Academy of Metrology and Quality Inspection (China)
Tao Xu, Shenzhen Academy of Metrology and Quality Inspection (China)

Published in SPIE Proceedings Vol. 6047:
Fourth International Conference on Photonics and Imaging in Biology and Medicine
Kexin Xu; Qingming Luo; Da Xing; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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