
Proceedings Paper
Dynamic measurement of fluorescent proteins spectral distribution on virus infected cellsFormat | Member Price | Non-Member Price |
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Paper Abstract
We constructed a dynamic spectroscopy system that can simultaneously measure the intensity and spectral distributions
of samples with multi-fluorophores in a single scan. The system was used to monitor the fluorescence distribution of
cells infected by the virus, which is constructed by a recombinant baculoviruses, vAcD-Rhir-E, containing the red and
green fluorescent protein gene that can simultaneously produce dual fluorescence in recombinant virus-infected
Spodoptera frugiperda 21 cells (Sf21) under the control of a polyhedrin promoter. The system was composed of an
excitation light source, a scanning system and a spectrometer. We also developed an algorithm and fitting process to
analyze the pattern of fluorescence distribution of the dual fluorescence produced in the recombinant virus-infected cells.
All the algorithm and calculation are automatically processed in a visualized scanning program and can monitor the
specific region of sample by calculating its intensity distribution. The spectral measurement of each pixel was performed
at millisecond range and the two dimensional distribution of full spectrum was recorded within several seconds. We have
constructed a dynamic spectroscopy system to monitor the process of virus-infection of cells. The distributions of the
dual fluorescence were simultaneously measured at micrometer resolution.
Paper Details
Date Published: 8 September 2006
PDF: 9 pages
Proc. SPIE 6343, Photonics North 2006, 63430E (8 September 2006); doi: 10.1117/12.707490
Published in SPIE Proceedings Vol. 6343:
Photonics North 2006
Pierre Mathieu, Editor(s)
PDF: 9 pages
Proc. SPIE 6343, Photonics North 2006, 63430E (8 September 2006); doi: 10.1117/12.707490
Show Author Affiliations
Ja-Yun Lee, Chung Yuan Christian Univ. (Taiwan)
Ming-Xiu Wu, Chung Yuan Christian Univ. (Taiwan)
Chia-Yun Kao, Chung Yuan Christian Univ. (Taiwan)
Ming-Xiu Wu, Chung Yuan Christian Univ. (Taiwan)
Chia-Yun Kao, Chung Yuan Christian Univ. (Taiwan)
Published in SPIE Proceedings Vol. 6343:
Photonics North 2006
Pierre Mathieu, Editor(s)
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