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Proceedings Paper

Multiphoton microscopy of atheroslcerotic plaques
Author(s): Magnus B. Lilledahl; Catharina de Lange Davies; Olav A. Haugen; Lars O. Svaasand
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Paper Abstract

Multiphoton microscopy is a techniques that fascilitates three dimensional imaging of intact, unstained tissue. Especially connective tissue has a relatively strong nonlinear optical response and can easily be imaged. Atherosclerosis is a disease where lipids accumulate in the vessel wall and there is a thickening of the intima by growth of a cap of connective tissue. The mechanical strength of this fibrous cap is of clinically importance. If the cap ruptures a thrombosis forms which can block a coronary vessel and therby causing myocardial infarction. Multiphoton microscopy can be used to image the fibrous cap and thereby determine the thickness of the cap and the structure of the connective fibres. This could possibly be developed into a diagnostic and clincal tool to monitor the vulnerability of a plaque and also to better understand the development of a plaque and effects of treatment. We have collected multiphoton microscopy images from atherosclerotic plaque in human aorta, both two photon excited fluorescens and second harmonic generated signal. The feasability of using this technique to determine the state of the plaque is explored.

Paper Details

Date Published: 23 March 2007
PDF: 9 pages
Proc. SPIE 6424, Photonic Therapeutics and Diagnostics III, 64241Y (23 March 2007); doi: 10.1117/12.706469
Show Author Affiliations
Magnus B. Lilledahl, Norwegian Univ. of Science and Technology (Norway)
Catharina de Lange Davies, Norwegian Univ. of Science and Technology (Norway)
Olav A. Haugen, Norwegian Univ. of Science and Technology (Norway)
St. Olavs Hospital (Norway)
Lars O. Svaasand, Norwegian Univ. of Science and Technology (Norway)

Published in SPIE Proceedings Vol. 6424:
Photonic Therapeutics and Diagnostics III
Henry Hirschberg M.D.; Brian Jet-Fei Wong M.D.; Reza S. Malek M.D.; Kenton W. Gregory M.D.; Nikiforos Kollias M.D.; Bernard Choi; Steen J. Madsen; Guillermo J. Tearney M.D.; Justus F. R. Ilgner M.D.; Haishan Zeng, Editor(s)

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