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Proceedings Paper

An integrated microsystem for multiplex processing of encoded silicon microbeads
Author(s): Daniel Hoffmann; Maeve Curtin; Michael Loughran
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Paper Abstract

A novel integrated microsystem for multiplex processing of encoded microbeads on a single microchip is presented. Conventional bio-analysis of proteins and DNA requires a combination of different techniques including: accurate delivery of reagents, mixing, then reaction at controlled temperature to yield a detectable product. Standard laboratory bio-assays require intervention at several stages to manipulate samples. Furthermore, ultra sensitive quantification with a colorimetric or fluorescent label is required to obtain the necessary results. This process is time consuming and labour intensive. However the new multiplex microsystem reported here enhances logical bio-assay development due to the integration of a compact optical detection system with customized analysis software in an enclosed microfluidic environment. The bioassay was realised by careful integration of a Peltier cell and associated control electronics which enabled specific identification of a hybridized DNA sequence from a 4 x 4 cDNA library at a fixed temperature of 42oC. The multiple fluorescence measurements of complementary hybridised DNA at the surface of encoded microbeads was confirmed. Thus, the complete integration of the different bio-assay components on a single multiplex assay platform provides distinct advantages of reduced sample volume, rapid analysis and low cost.

Paper Details

Date Published: 22 January 2007
PDF: 8 pages
Proc. SPIE 6465, Microfluidics, BioMEMS, and Medical Microsystems V, 64650D (22 January 2007);
Show Author Affiliations
Daniel Hoffmann, Univ. College Cork (Ireland)
Maeve Curtin, Univ. College Cork (Ireland)
Michael Loughran, Univ. College Cork (Ireland)

Published in SPIE Proceedings Vol. 6465:
Microfluidics, BioMEMS, and Medical Microsystems V
Ian Papautsky; Wanjun Wang, Editor(s)

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